Brij-58 supplementation enhances menaquinone-7 biosynthesis and secretion in Bacillus natto.

Autor: Yi Y; School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China., Jin X; School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China., Chen M; Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China., Coldea TE; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, 400372, Cluj-Napoca-Napoca, Romania., Yang H; College of Food Science and Technology, Southwest Minzu University, Chengdu, 610041, China. zyjyhr@sina.com., Zhao H; School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China. hfzhao@scut.edu.cn.
Jazyk: angličtina
Zdroj: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Aug; Vol. 107 (16), pp. 5051-5062. Date of Electronic Publication: 2023 Jun 26.
DOI: 10.1007/s00253-023-12640-y
Abstrakt: Menaquinone-7 is a form of vitamin K 2 that has been shown to have numerous healthy benefits. In this study, several surfactants were investigated to enhance the production of menaquinone-7 in Bacillus natto. Results showed that Brij-58 supplementation influenced the cell membrane via adsorption, and changed the interfacial tension of fermentation broth, while the changes in the state and the composition of the cell membrane enhanced the secretion and biosynthesis of menaquinone-7. The total production and secretion rate of menaquinone-7 increased by 48.0% and 56.2% respectively. During fermentation, the integrity of the cell membrane decreased by 82.9% while the permeability increased by 158% when the maximum secretory rate was reached. Furthermore, Brij-58 supplementation induced the stress response in bacteria, resulting in hyperpolarization of the membrane, and increased membrane ATPase activity. Finally, changes in fatty acid composition increased membrane fluidity by 30.1%. This study provided an effective strategy to enhance menaquinone-7 yield in Bacillus natto and revealed the mechanism of Brij-58 supplementation in menaquinone-7 production. KEY POINTS: • MK-7 yield in Bacillus natto was significantly increased by Brij-58 supplementation. • Brij-58 could be adsorbed on cell surface and change fermentation environment. • Brij-58 supplementation could affect the state and composition of the cell membrane.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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