The effects of ketamine on viability, primary DNA damage, and oxidative stress parameters in HepG2 and SH-SY5Y cells.

Autor:	Jurič A; 1Institute for Medical Research and Occupational Health, Zagreb, Croatia., Lovaković BT; 1Institute for Medical Research and Occupational Health, Zagreb, Croatia., Zandona A; 1Institute for Medical Research and Occupational Health, Zagreb, Croatia., Rašić D; 1Institute for Medical Research and Occupational Health, Zagreb, Croatia., Češi M; 2University of Zagreb Faculty of Food Technology and Biotechnology, Zagreb, Croatia., Pizent A; 1Institute for Medical Research and Occupational Health, Zagreb, Croatia., Neuberg M; 3University North, University Centre Varaždin, Varaždin, Croatia., Canjuga I; 3University North, University Centre Varaždin, Varaždin, Croatia., Katalinić M; 1Institute for Medical Research and Occupational Health, Zagreb, Croatia., Vrdoljak AL; 1Institute for Medical Research and Occupational Health, Zagreb, Croatia., Rešić A; 4Children's Hospital Zagreb Department of Paediatrics, Zagreb, Croatia., Karačonji IB; 1Institute for Medical Research and Occupational Health, Zagreb, Croatia.; 5University of Rijeka Faculty of Health Studies, Rijeka, Croatia.
Jazyk:	angličtina
Zdroj:	Arhiv za higijenu rada i toksikologiju [Arh Hig Rada Toksikol] 2023 Jun 26; Vol. 74 (2), pp. 106-114. Date of Electronic Publication: 2023 Jun 26 (Print Publication: 2023).
DOI:	10.2478/aiht-2023-74-3727
Abstrakt:	Ketamine is a dissociative anaesthetic used to induce general anaesthesia in humans and laboratory animals. Due to its hallucinogenic and dissociative effects, it is also used as a recreational drug. Anaesthetic agents can cause toxic effects at the cellular level and affect cell survival, induce DNA damage, and cause oxidant/antioxidant imbalance. The aim of this study was to explore these possible adverse effects of ketamine on hepatocellular HepG2 and neuroblastoma SH-SY5Y cells after 24-hour exposure to a concentration range covering concentrations used in analgesia, drug abuse, and anaesthesia (0.39, 1.56, and 6.25 µmol/L, respectively). At these concentrations ketamine had relatively low toxic outcomes, as it lowered HepG2 and SH-SY5Y cell viability up to 30 %, and low, potentially repairable DNA damage. Interestingly, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH) remained unchanged in both cell lines. On the other hand, oxidative stress markers [superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT)] pointed to ketamine-induced oxidant/antioxidant imbalance. (© 2023 Andreja Jurič et al., published by Sciendo.)
Databáze:	MEDLINE
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