The HIV-1 capsid core is an opportunistic nuclear import receptor.

Autor: Xue G; Model Development Section, Cancer Innovation Laboratory, National Cancer Institute, Frederick, MD, 21702, USA., Yu HJ; Basic Science Program, Leidos Biomedical Research, Frederick National Laboratory, Frederick, MD, 21702, USA., Buffone C; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, 10461, USA., Huang SW; Model Development Section, Cancer Innovation Laboratory, National Cancer Institute, Frederick, MD, 21702, USA., Lee K; Model Development Section, Cancer Innovation Laboratory, National Cancer Institute, Frederick, MD, 21702, USA., Goh SL; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USA., Gres AT; Bond Life Sciences Center, Chemistry, University of Missouri, Columbia, MO, 65201, USA., Guney MH; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USA., Sarafianos SG; Bond Life Sciences Center, Chemistry, University of Missouri, Columbia, MO, 65201, USA.; Bond Life Sciences Center, MMI, Biochemistry, University of Missouri, Columbia, MO, 65201, USA.; Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA., Luban J; Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, 01605, USA., Diaz-Griffero F; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, 10461, USA., KewalRamani VN; Model Development Section, Cancer Innovation Laboratory, National Cancer Institute, Frederick, MD, 21702, USA. vineet@mail.nih.gov.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Jun 24; Vol. 14 (1), pp. 3782. Date of Electronic Publication: 2023 Jun 24.
DOI: 10.1038/s41467-023-39146-5
Abstrakt: The movement of viruses and other large macromolecular cargo through nuclear pore complexes (NPCs) is poorly understood. The human immunodeficiency virus type 1 (HIV-1) provides an attractive model to interrogate this process. HIV-1 capsid (CA), the chief structural component of the viral core, is a critical determinant in nuclear transport of the virus. HIV-1 interactions with NPCs are dependent on CA, which makes direct contact with nucleoporins (Nups). Here we identify Nup35, Nup153, and POM121 to coordinately support HIV-1 nuclear entry. For Nup35 and POM121, this dependence was dependent cyclophilin A (CypA) interaction with CA. Mutation of CA or removal of soluble host factors changed the interaction with the NPC. Nup35 and POM121 make direct interactions with HIV-1 CA via regions containing phenylalanine glycine motifs (FG-motifs). Collectively, these findings provide additional evidence that the HIV-1 CA core functions as a macromolecular nuclear transport receptor (NTR) that exploits soluble host factors to modulate NPC requirements during nuclear invasion.
(© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze: MEDLINE
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