Engineering Cell Lines for Specific Human Leukocyte Antigen Presentation.

Autor:	Jin D; Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, VIC, Australia., Loh KL; Centre for Inflammatory Diseases, Department of Medicine, Monash University, Monash Medical Centre, Clayton, VIC, Australia., Shamekhi T; Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, VIC, Australia., Ting YT; Centre for Inflammatory Diseases, Department of Medicine, Monash University, Monash Medical Centre, Clayton, VIC, Australia., Lim Kam Sian TCC; Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, VIC, Australia.; Monash Proteomics & Metabolomics Facility, Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia., Roest J; St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia., Ooi JD; Centre for Inflammatory Diseases, Department of Medicine, Monash University, Monash Medical Centre, Clayton, VIC, Australia., Vivian JP; St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia. jvivian@svi.edu.au.; Department of Medicine, The University of Melbourne, Melbourne, VIC, Australia. jvivian@svi.edu.au., Faridi P; Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, VIC, Australia.; Monash Proteomics & Metabolomics Facility, Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2691, pp. 351-369.
DOI:	10.1007/978-1-0716-3331-1_25
Abstrakt:	Epitope-specific immunotherapies have enabled the targeted treatment of a variety of diseases, ranging from cancer, infection, and autoimmune disorders. For CD8 + T cell-based therapies, the precise identification of immunogenic peptides presented by human leukocyte antigen (HLA) class I is essential which can be achieved by immunopeptidomics. Here, using lentivirus-mediated transduction and cell sorting approaches, we present a method to engineer a cell line that does not express its native HLA but instead expresses an HLA of interest (in this instance HLA-A*02:01). This technique can be used to elucidate the immunopeptidome of cell lines expressing different HLAs. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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