Virulence Signatures, Integrons, and Antibiotic Resistance Genes in Bacterial Strains Recovered from Selected Commercial Dairy Products and Fresh Raw Meat.

Autor: Aiyegoro OA; Collaborating Partner, Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Nigeria.; Research Unit for Environmental Sciences and Management, North West University, Potchefstroom, 2520, North West, South Africa., Moyane JN; School of Agriculture, Department of Food Science and Technology, University of Venda, Thohoyandou, 0950, South Africa., Adegoke AA; Department of Microbiology, Faculty of Science, University of Uyo, Uyo, Nigeria. anthonyadegoke@uniuyo.edu.ng.; Department of Community Health Studies, Faculty of Health Sciences, Durban University of Technology (DUT), Durban, 4001, KwaZulu-Natal, South Africa. anthonyadegoke@uniuyo.edu.ng., Jideani AIO; School of Agriculture, Department of Food Science and Technology, University of Venda, Thohoyandou, 0950, South Africa.; Postharvest-Handling Group, ISEKI-Food Association, Vienna, Austria., Reddy P; Department of Community Health Studies, Faculty of Health Sciences, Durban University of Technology (DUT), Durban, 4001, KwaZulu-Natal, South Africa., Okoh AI; Department of Environmental Health Sciences, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.; SAMRC Microbial Water Quality Monitoring Centre, University of For Hare, Alice, South Africa.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2023 Jun 24; Vol. 80 (8), pp. 254. Date of Electronic Publication: 2023 Jun 24.
DOI: 10.1007/s00284-023-03371-4
Abstrakt: Bacterial species responsible for food infections and intoxication are sometimes carried through the food production and processing. Very few published literatures exist on integrons among antibiotic-resistant staphylococcal strains from foods of animal origin in Gauteng Province, South Africa, hence this study. A total of 720 samples (360 meat and 360 dairies) from a community abattoir of a research farm in South Africa, using conventional bacteriological and molecular methods. Nine (9) bacterial strains, including Bacillus subtilis AYO-123, Acinetobacter baumannii AYO-241, Staphylococcus lentus AYO-352, among others were identified and submitted to GenBank. More bacterial strains were recovered from raw meat (90.5%) than dairy products (9.5%). Resistance was shown (0-100%) to Imipenem, Meropenem, Norfloxacin, Clindamycin, and 22 other antibiotics, without any carbapenem-resistant Acinetobacter baumannii and methicillin/vancomycin-resistant Staphylococcus species (MRSS/VRSS). Virulence genes for fibronectin-binding protein A (FnbA) were predominant (56.24%) followed by the circulating nucleic acids (cna) gene (43.75%). Others were staphylococcal enterotoxin A (sea, 41%), staphylococcal enterotoxin B (seb, 23.5%). Co-presence of sea and seb genes occurred in 11.76% of the isolates, but no coa genes was amplified. Antibiotic resistance genes (ARGs), tetK (70.58%), linA (29.4%), and ermA (11.76%) were detected, but none of the mecA and vat genes was amplified. Class 2 integron (50%) was more predominantly detected than integron 1 (25%), but no Class 3 integron was detected. Bacteria with both the detected virulence and antibiotic resistance genes are of potential risks to human health.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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