Self-Assembly of Platelet Lysates Proteins into Microparticles by Unnatural Disulfide Bonds for Bottom-Up Tissue Engineering.

Autor: Gomes MC; Department of Chemistry CICECO-Aveiro Institute of Materials University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal., Pinho AR; Department of Chemistry CICECO-Aveiro Institute of Materials University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal., Custódio C; Department of Chemistry CICECO-Aveiro Institute of Materials University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal., Mano JF; Department of Chemistry CICECO-Aveiro Institute of Materials University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2023 Oct; Vol. 35 (41), pp. e2304659. Date of Electronic Publication: 2023 Aug 24.
DOI: 10.1002/adma.202304659
Abstrakt: There is a demand to design microparticles holding surface topography while presenting inherent bioactive cues for applications in the biomedical and biotechnological fields. Using the pool of proteins present in human-derived platelet lysates (PLs), the production of protein-based microparticles via a simple and cost-effective method is reported, exploring the prone redox behavior of cysteine (Cy-SH) amino acid residues. The forced formation of new intermolecular disulfide bonds results in the precipitation of the proteins as spherical, pompom-like microparticles with adjustable sizes (15-50 µm in diameter) and surface topography consisting of grooves and ridges. These PL microparticles exhibit extraordinary cytocompatibility, allowing cell-guided microaggregates to form, while also working as injectable systems for cell support. Early studies also suggest that the surface topography provided by these PL microparticles can support osteogenic behavior. Consequently, these PL microparticles may find use to create live tissues via bottom-up procedures or injectable tissue-defect fillers, particularly for bone regeneration, with the prospect of working under xeno-free conditions.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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