Simultaneous quantitation of urine aldosterone and tetrahydroaldosterone in healthy Chinese subjects using a validated LC-MS/MS method.

Autor: Cheng JYK; Department of Chemical Pathology, Prince of Wales Hospital, Hong Kong, Hong Kong., Lo CWS; Department of Chemical Pathology, Prince of Wales Hospital, Hong Kong, Hong Kong., Chan ASL; Department of Chemical Pathology, Prince of Wales Hospital, Hong Kong, Hong Kong., Luk YK; Department of Chemical Pathology, Prince of Wales Hospital, Hong Kong, Hong Kong., Tsui TKC; Department of Chemical Pathology, Prince of Wales Hospital, Hong Kong, Hong Kong., Ho CS; Department of Chemical Pathology, The Chinese University of Hong Kong, Ma Liu Shui, Hong Kong.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2023 Sep; Vol. 37 (9), pp. e5694. Date of Electronic Publication: 2023 Jun 24.
DOI: 10.1002/bmc.5694
Abstrakt: Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an alternative screening test to plasma ALD for primary aldosteronism. We report a validated LC-MS/MS method to measure both analytes simultaneously. Urine samples underwent enzymatic hydrolysis to release the analytes from their glucuronide conjugates followed by organic solvent extraction and LC-MS/MS. The analytical performance of this method was evaluated. The within-batch and between-batch coefficients of variation for urine ALD and urine THA were all ≤5.2 and ≤3.7%. The lower limit of quantification was 0.5 nmol/L, and the linearity was up to at least 2770 nmol/L for both analytes. No significant matrix interference and carryover were observed. Both analytes in urine were stable for at least 48 h at 10°C and at least 18 months at -80°C. Local reference intervals were established from 126 healthy normotensive Chinese subjects (53% women, age: 20-65 years). Reference intervals for urine ALD and tetrahydroaldosterone were 2-38 and 9-139 nmol/day, respectively. This validated method can be applied to screening and diagnosing primary aldosteronism.
(© 2023 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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