Nitrate Reductase Assay for Rapid Determination of Methicillin-Resistant Staphylococcus aureus Clinical Isolates.

Autor:	Yildirim K; Akdeniz University Tuberculosis Research Center, Antalya, Türkiye.; Department of Nutrition and Dietetics, Faculty of Health Sciences, Akdeniz University, Antalya, Türkiye., Atas C; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Türkiye., Tanyel Akcit E; Akdeniz University Tuberculosis Research Center, Antalya, Türkiye.; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Türkiye., Licina M; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Türkiye., Koyuncu Ozyurt O; Department of Medical Microbiology, Akdeniz University, Antalya, Türkiye., Gun MA; 19 Mayis State Hospital, Samsun, Türkiye., Simsek E; Akdeniz University Tuberculosis Research Center, Antalya, Türkiye.; Department of Nutrition and Dietetics, Faculty of Health Sciences, Akdeniz University, Antalya, Türkiye.; Department of Medical Microbiology, Akdeniz University, Antalya, Türkiye., Coban AY; Akdeniz University Tuberculosis Research Center, Antalya, Türkiye.; Department of Nutrition and Dietetics, Faculty of Health Sciences, Akdeniz University, Antalya, Türkiye.; Department of Medical Microbiology, Akdeniz University, Antalya, Türkiye.
Jazyk:	angličtina
Zdroj:	Laboratory medicine [Lab Med] 2024 Mar 07; Vol. 55 (2), pp. 174-178.
DOI:	10.1093/labmed/lmad056
Abstrakt:	Objective: To evaluate the performance of nitrate reductase assay (NRA), a rapid, colorimetric method for the determination of methicillin resistance in Staphylococcus aureus isolates obtained from the culture collection of the Akdeniz University Hospital Central Laboratory, Antalya, Türkiye. Materials and Methods: Identification for all 290 S aureus isolates at the species level was performed via matrix-assisted laser desorption/ionization-time of flight. Isolates were tested with NRA for methicillin resistance. The cefoxitin broth microdilution (BMD) method recommended by the Clinical and Laboratory Standards Institute was used as the reference method in the study. S aureus ATCC 29213 and S aureus ATCC 43300 strains were used for quality control. Results: According to Food and Drug Administration criteria, the category agreement between NRA and BMD was found to be 100%. The essential agreement between both methods was determined to be 96.20%. There is no minor, major, or extremely major discrepancy between both methods. Conclusion: The results show that NRA is a rapid, practical, and reliable colorimetric method for detecting MRSA. (© The Author(s) 2023. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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