Leaf spectroscopy of resistance to Ceratocystis wilt of 'Ōhi'a.

Autor: Seeley MM; Center for Global Discovery and Conservation Science, Arizona State University, Hilo, Hawai'i, United States of America.; School of Geographical Sciences and Urban Planning, Arizona State University, Tempe, Arizona, United States of America., Martin RE; Center for Global Discovery and Conservation Science, Arizona State University, Hilo, Hawai'i, United States of America.; School of Geographical Sciences and Urban Planning, Arizona State University, Tempe, Arizona, United States of America., Giardina C; Institute of Pacific Islands Forestry, Pacific Southwest Research Station, USDA Forest Service, Hilo, Hawai'i, United States of America., Luiz B; Akaka Foundation for Tropical Forests, Hilo, Hawai'i, United States of America., Francisco K; Institute of Pacific Islands Forestry, Pacific Southwest Research Station, USDA Forest Service, Hilo, Hawai'i, United States of America., Cook Z; Institute of Pacific Islands Forestry, Pacific Southwest Research Station, USDA Forest Service, Hilo, Hawai'i, United States of America., Hughes MA; Institute of Pacific Islands Forestry, Pacific Southwest Research Station, USDA Forest Service, Hilo, Hawai'i, United States of America., Asner GP; Center for Global Discovery and Conservation Science, Arizona State University, Hilo, Hawai'i, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Jun 23; Vol. 18 (6), pp. e0287144. Date of Electronic Publication: 2023 Jun 23 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0287144
Abstrakt: Plant pathogens are increasingly compromising forest health, with impacts to the ecological, economic, and cultural goods and services these global forests provide. One response to these threats is the identification of disease resistance in host trees, which with conventional methods can take years or even decades to achieve. Remote sensing methods have accelerated host resistance identification in agricultural crops and for a select few forest tree species, but applications are rare. Ceratocystis wilt of 'ōhi'a, caused by the fungal pathogen Ceratocystis lukuohia has been killing large numbers of the native Hawaiian tree, Metrosideros polymorpha or 'Ōhi'a, Hawaii's most common native tree and a biocultural keystone species. Here, we assessed whether resistance to C. lukuohia is detectable in leaf-level reflectance spectra (400-2500 nm) and used chemometric conversion equations to understand changes in leaf chemical traits of the plants as indicators of wilt symptom progression. We collected leaf reflectance data prior to artificially inoculating 2-3-year-old M. polymorpha clones with C. lukuohia. Plants were rated 3x a week for foliar wilt symptom development and leaf spectra data collected at 2 to 4-day intervals for 120 days following inoculation. We applied principal component analysis (PCA) to the pre-inoculation spectra, with plants grouped according to site of origin and subtaxon, and two-way analysis of variance to assess whether each principal component separated individuals based on their disease severity ratings. We identified seven leaf traits that changed in susceptible plants following inoculation (tannins, chlorophyll a+b, NSC, total C, leaf water, phenols, and cellulose) and leaf chemistries that differed between resistant and early-stage susceptible plants, most notably chlorophyll a+b and cellulose. Further, disease resistance was found to be detectable in the reflectance data, indicating that remote sensing work could expedite Ceratocystis wilt of 'ōhi'a resistance screenings.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.)
Databáze: MEDLINE
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