Validation and Application of UPLC-MS/MS Method to Analysis of Glyphosate and its Metabolites in Water.

Autor:	de J Bastidas-Bastidas P; Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a Eldorado Km. 5.5 Campo el Diez, Culiacán, C.P. 80110 Sinaloa, México., Cabrera R; Conahcyt-Centro de Investigación en Alimentación y Desarrollo, A.C., Unidad Regional Hidalgo, Distrito de Educación, Salud, Ciencia, Tecnología e Innovación de Hidalgo, Blvd. Santa Catarina, San Agustín Tlaxiaca, Hidalgo, C.P. 42163, Mexico., Valenzuela-Quintanar AI; Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46 Colonia La Victoria, Hermosillo, Sonora C.P. 83304, México., Olmeda-Rubio C; Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a Eldorado Km. 5.5 Campo el Diez, Culiacán, C.P. 80110 Sinaloa, México., González-Mendoza VM; Conahcyt-Centro de Investigación en Alimentación y Desarrollo, A.C., Unidad Regional Hidalgo, Distrito de Educación, Salud, Ciencia, Tecnología e Innovación de Hidalgo, Blvd. Santa Catarina,San Agustín Tlaxiaca, Hidalgo, C.P. 42163, Mexico., Perea-Domínguez XP; Doctorado en Sustentabilidad, Universidad Autónoma de Occidente, Unidad Regional Guasave, Av Universidad S/N, Fraccionamiento Villa Universidad, Guasave, Sinaloa C.P. 81048, México., González-Márquez LC; Doctorado en Sustentabilidad, Universidad Autónoma de Occidente, Unidad Regional Guasave, Av Universidad S/N, Fraccionamiento Villa Universidad, Guasave, Sinaloa C.P. 81048, México., Del C Salvatierra-Stamp V; Facultad de Ciencias Químicas, Universidad de Colima, Carretera Colima-Coquimatlán km 9, Coquimatlán, Colima C.P. 28400, México., Leyva-Morales JB; Centro de Investigación en Recursos Naturales y Sustentabilidad (CIRENYS), Universidad Bernardo O'Higgins, Avenida Viel 1497, Santiago de C.P. 8370993, Chile.; Universidad Politécnica del Mar y la Sierra. Carretera a Potrerillos del Norote km.3, La Cruz, Elota, Sinaloa C.P. 82700, Mexico.
Jazyk:	angličtina
Zdroj:	Journal of chromatographic science [J Chromatogr Sci] 2024 Apr 23; Vol. 62 (4), pp. 364-371.
DOI:	10.1093/chromsci/bmad045
Abstrakt:	A method was developed to determine glyphosate and their metabolites in water. The widespread use of this herbicide in agricultural activities worldwide, despite the reported adverse effects on both the environment and health, is a cause for concern and makes it necessary to monitor its presence through a method that guarantees the determination at trace levels. A direct extraction of the analytes with phosphate buffer was performed with subsequent derivatization with 9-fluorenylmethyl chloroformate. The quantification was determined by Ultra Performance Liquid Chromatography-tandem mass spectrometer. The method was validated through the following parameters: selectivity, detection and quantification limits, linearity, accuracy, precision and uncertainty. The average recoveries ranged between 94.08 and 103.31%. Additionally, detection limits from 0.396 to 0.433 μg/L, and the quantification limit was 5.0 μg/L for all the analytes evaluated. In terms of linearity and precision, the results obtained were in the ranges considered adequate (R2 ≥ 0.99 and CV ≤ 20%), the estimated expanded uncertainty was 12.95, 11.15 and 13.83% for glyphosate, aminomethylphosphonic acid and glufosinate, respectively. This method was successfully applied for the determination of the target analytes in irrigation water samples, detecting concentrations of aminomethylphosphonic acid over limit detection for some sampling sites. (© The Author(s) 2023. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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