| Autor: |
de Melo Alcântara LF; Programa de Pós-Graduação Em Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, Brasil., da Silva PT; Programa de Pós-Graduação Em Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, Brasil., de Oliveira QM; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE, Brasil., Dos Santos Souza TG; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE, Brasil., da Silva MM; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE, Brasil., Feitoza GS; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, Brasil., Costa WK; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, Brasil., da Conceição de Lira MA; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE, Brasil., Chagas CA; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE, Brasil., de Aguiar Júnior FCA; Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE, Brasil., Dos Santos Correia MT; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, Brasil., da Silva MV; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, Brasil. |
| Abstrakt: |
This study aimed to characterize the phytochemical profile of bark and leaves aqueous extract Commiphora leptophloeos , and conduct in vivo and in vitro assays to determine the presence of any toxicological consequences due to exposure. The phytochemical analysis was carried out using high-performance liquid chromatography (HPLC). The antioxidant activity was estimated utilizing DPPH free radical scavenging and phosphomolybdenum assays. Cell viability was measured by the MTT method on J774 and human adenocarcinoma cells, which were treated with concentrations of 12,5, 25, 50, 100 or 200 µg/ml of both extracts. Acute oral toxicity, genotoxicity, and mutagenicity assays were determined using a single oral dose of 2000 g/kg in male Swiss albino mice ( Mus musculus ). Biochemical analysis of the blood and histological analyses of the kidneys, liver, spleen, pylorus, duodenum and jejunum were undertaken. Genotoxicity and mutagenicity were determined utilizing blood samples. Gallic acid, catechin, and epicatechin were identified in the bark and chlorogenic acid in leaves. Data demonstrated a high content of phenolic compounds and flavonoids associated with significant antioxidant potential. No significant signs in damage or symptoms of toxicity were detected. No marked reduction in cell viability was found at lower concentrations tested. On histomorphometry, only the gastrointestinal organs exhibited significant difference. Renal hepatic and blood parameters were within the normal range. No apparent signs of toxicity, genotoxicity, mutagenicity or cytotoxicity were found in vivo and in vitro experiments. |
