Isolation and characterization of the gene HvFAR1 encoding acyl-CoA reductase from the cer-za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions.

Autor: Müller Y; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany., Patwari P; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany., Stöcker T; Institute of Crop Science and Resource Conservation, University of Bonn, 53115, Bonn, Germany., Zeisler-Diehl V; Institute for Cellular and Molecular Botany, University of Bonn, 53115, Bonn, Germany., Steiner U; Institute of Crop Science and Resource Conservation, University of Bonn, 53115, Bonn, Germany., Campoli C; Division of Plant Sciences, School of Life Sciences, University of Dundee, Dundee, DD2 5DA, UK.; James Hutton Institute, Dundee, DD2 5DA, UK., Grewe L; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany., Kuczkowska M; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany., Dierig MM; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany., Jose S; School of Biological Sciences, University of Bristol, Bristol, BS8 1TQ, UK., Hetherington AM; School of Biological Sciences, University of Bristol, Bristol, BS8 1TQ, UK., Acosta IF; Max-Planck Institute for Plant Breeding Research, 50829, Cologne, Germany., Schoof H; Institute of Crop Science and Resource Conservation, University of Bonn, 53115, Bonn, Germany., Schreiber L; Institute for Cellular and Molecular Botany, University of Bonn, 53115, Bonn, Germany., Dörmann P; Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115, Bonn, Germany.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2023 Sep; Vol. 239 (5), pp. 1903-1918. Date of Electronic Publication: 2023 Jun 22.
DOI: 10.1111/nph.19063
Abstrakt: The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer-za.227 and cer-ye.267 display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown. Cuticular waxes and permeabilities were measured in cer-za.227 and cer-ye.267. The mutant loci were isolated by bulked segregant RNA sequencing. New cer-za alleles were generated by genome editing. The CER-ZA protein was characterized after expression in yeast and Arabidopsis cer4-3. Cer-za.227 carries a mutation in HORVU5Hr1G089230 encoding acyl-CoA reductase (FAR1). The cer-ye.267 mutation is located to HORVU4Hr1G063420 encoding β-ketoacyl-CoA synthase (KAS1) and is allelic to cer-zh.54. The amounts of intracuticular waxes were strongly decreased in cer-ye.267. The cuticular water loss and permeability of cer-za.227 were similar to wild-type (WT), but were increased in cer-ye.267. Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration. The differential decrease in intracuticular waxes between cer-za.227 and cer-ye.267, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes.
(© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.)
Databáze: MEDLINE
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