Thiol functionalised gold nanoparticles loaded with methotrexate for cancer treatment: From synthesis to in vitro studies on neuroblastoma cell lines.

Autor: Salamone TA; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy. Electronic address: tommaso.salamone@uniroma1.it., Rutigliano L; Department of Molecular Medicine, Laboratory Affiliated to Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, Italy., Pennacchi B; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy., Cerra S; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy., Matassa R; Department of Anatomical, Histological, Forensic and Orthopaedic Sciences, Section of Human Anatomy, Sapienza University of Rome, Via A. Borelli 50, 00161 Rome, Italy., Nottola S; Department of Anatomical, Histological, Forensic and Orthopaedic Sciences, Section of Human Anatomy, Sapienza University of Rome, Via A. Borelli 50, 00161 Rome, Italy., Sciubba F; Department of Environmental Biology, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy; NMR-based Metabolomics Laboratory (NMLab), Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy., Battocchio C; Department of Sciences, Roma Tre University, Via della Vasca Navale 79, 00146 Rome, Italy., Marsotto M; Department of Sciences, Roma Tre University, Via della Vasca Navale 79, 00146 Rome, Italy., Del Giudice A; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy., Chumakov A; Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany., Davydok A; Institute of Material Physics, Helmholtz Zentrum Hereon, Notkestr. 85, 22607 Hamburg, Germany., Grigorian S; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy; Institute of Physics, University of Siegen, Walter-Flex-Strasse 3, D-57068 Siegen, Germany., Canettieri G; Department of Molecular Medicine, Laboratory Affiliated to Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Sapienza University of Rome, Viale Regina Elena 291, 00161 Rome, Italy., Agostinelli E; Department of Sense Organs, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy; International Polyamines Foundation 'ETS-ONLUS', Via del Forte Tiburtino 98, 00159 Rome, Italy., Fratoddi I; Department of Chemistry, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, Italy. Electronic address: ilaria.fratoddi@uniroma1.it.
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2023 Nov; Vol. 649, pp. 264-278. Date of Electronic Publication: 2023 Jun 16.
DOI: 10.1016/j.jcis.2023.06.078
Abstrakt: Hypothesis: Colloidal gold nanoparticles (AuNPs) functionalised with hydrophilic thiols can be used as drug delivery probes, thanks to their small size and hydrophilic character. AuNPs possess unique properties for their use in nanomedicine, especially in cancer treatment, as diagnostics and therapeutic tools.
Experiments: Thiol functionalised AuNPs were synthesised and loaded with methotrexate (MTX). Spectroscopic and morphostructural characterisations evidenced the stability of the colloids upon interaction with MTX. Solid state (GISAXS, GIWAXS, FESEM, TEM, FTIR-ATR, XPS) and dispersed phase (UV-Vis, DLS, ζ-potential, NMR, SAXS) experiments allowed to understand structure-properties correlations. The nanoconjugate was tested in vitro (MTT assays) against two neuroblastoma cell lines: SNJKP and IMR5 with overexpressed n-Myc.
Findings: Molar drug encapsulation efficiency was optimised to be >70%. A non-covalent interaction between the π system and the carboxylate moiety belonging to MTX and the charged aminic group of one of the thiols was found. The MTX loading slightly decreased the structural order of the system and increased the distance between the AuNPs. Free AuNPs showed no cytotoxicity whereas the AuNPs-MTX nanoconjugate had a more potent effect when compared to free MTX. The active role of AuNPs was evidenced by permeation studies: an improvement on penetration of the drug inside cells was evidenced.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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