Comparative Diagnostic of Cervical Tuberculous Lymphadenitis: PCR is a Fast, Efficient, and Improved Diagnostic Approach.

Autor: Jha H; Department of Microbiology, Maulana Azad Medical College, New Delhi 110002, India.; Lab Strengthening & Diagnostics, Jhpiego, India., Baveja CP; Department of Microbiology, Maulana Azad Medical College, New Delhi 110002, India., Kamal V; Department of Pathology, Maulana Azad Medical College, New Delhi 110002, India., Agarwal PN; Department of Surgery, Maulana Azad Medical College, New Delhi 110002, India., Saxena S; Department of Microbiology, Maulana Azad Medical College, New Delhi 110002, India., Dhakad MS; Department of Microbiology, Maulana Azad Medical College, New Delhi 110002, India., Sharma D; Department of Microbiology, Lady Hardinge Medical College, New Delhi 110001, India.
Jazyk: angličtina
Zdroj: The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale [Can J Infect Dis Med Microbiol] 2023 Jun 13; Vol. 2023, pp. 3312250. Date of Electronic Publication: 2023 Jun 13 (Print Publication: 2023).
DOI: 10.1155/2023/3312250
Abstrakt: Methods: The study included 100 clinically suspected cases of TBLN. Fine needle aspirate (FNA) samples were processed for cytology staining and cultured on LJ & BACTEC 12B media. The biochemical tests were performed to identify the isolates at the species level. Additionally, for PCR, DNA was extracted and used for the diagnosis and identification of mycobacterial species.
Results: Patients ranged from 2 to 45 years with a mean age of 24.96 ± 9.10 years. Out of 100 patients, 73% had clinical symptoms of weight loss, followed by fever (72%), anorexia (66%), and night sweats (58%). 24% of patients were found to be smear-positive after Ziehl-Neelsen (ZN) staining and statistically highly significant with PCR. On LJ medium 34% and on BACTEC radiometric 45% of samples were smearing positive. Overall, 48% of cases were PCR-positive for TBLN. When compared with culture, the sensitivity and specificity of PCR were 93.75% and 100%, respectively, which are higher than cytology. The true positive predictive value (PPV) and negative predictive value (NPV) were 83.3% and 61.5%, respectively.
Conclusion: This study suggests that PCR is a rapid, sensitive, and specific tool for correct diagnosis of TBLN cases as compared to staining and culture which lead to the early and proper management of mycobacterial diseases.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2023 Himanshu Jha et al.)
Databáze: MEDLINE
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