Natural antisense transcription of presenilin in sea urchin reveals a possible role for natural antisense transcription in the general control of gene expression during development

Autor:	Bronchain O; Paris-Saclay Institute of Neuroscience, CNRS, UMR CNRS 9197, Université Paris-Saclay, 75005 Paris, France., Ducos B; High Throughput qPCR Core Facility of the ENS, Université PSL, IBENS, Laboratoire de Physique de l'Ecole normale supérieure, ENS, Université PSL, CNRS, Sorbonne Université, Université de Paris, 75005 Paris, France., Putzer H; CNRS, Université Paris Cité, Expression Génétique Microbienne, IBPC, 75005 Paris, France., Delagrange M; High Throughput qPCR Core Facility of the ENS, Université PSL, IBENS, Laboratoire de Physique de l'Ecole normale supérieure, ENS, Université PSL, CNRS, Sorbonne Université, Université de Paris, 75005 Paris, France., Laalami S; CNRS, Université Paris Cité, Expression Génétique Microbienne, IBPC, 75005 Paris, France., Philippe-Caraty L; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 91190 Gif-sur-Yvette, France., Saroul K; Institut CURIE, Université Paris-Saclay, INSERM U932, Immunité et Cancer, 91400 Orsay, France., Ciapa B; Paris-Saclay Institute of Neuroscience, CNRS, UMR CNRS 9197, Université Paris-Saclay, 75005 Paris, France.
Jazyk:	angličtina
Zdroj:	Journal of cell science [J Cell Sci] 2023 Jul 15; Vol. 136 (14). Date of Electronic Publication: 2023 Jul 25.
DOI:	10.1242/jcs.261284
Abstrakt:	One presenilin gene (PSEN) is expressed in the sea urchin embryo, in the vegetal pole of the gastrula and then mainly in cilia cells located around the digestive system of the pluteus, as we recently have reported. PSEN expression must be accurately regulated for correct execution of these two steps of development. While investigating PSEN expression changes in embryos after expansion of endoderm with LiCl or of ectoderm with Zn2+ by whole-mount in situ hybridization (WISH) and quantitative PCR (qPCR), we detected natural antisense transcription of PSEN. We then found that Endo16 and Wnt5, markers of endo-mesoderm, and of Hnf6 and Gsc, markers of ectoderm, are also sense and antisense transcribed. We discuss that general gene expression could depend on both sense and antisense transcription. This mechanism, together with the PSEN gene, should be included in gene regulatory networks (GRNs) that theorize diverse processes in this species. We suggest that it would also be relevant to investigate natural antisense transcription of PSEN in the field of Alzheimer's disease (AD) where the role of human PSEN1 and PSEN2 is well known. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2023. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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