A Novel Approach to Study Coherent γ-Band Oscillations in Hippocampal Brain Sections.

Autor:	Rodríguez Díaz JC; Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, 48109 MI., Jenkins PM; Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, 48109 MI.; Department of Pharmacology, University of Michigan Medical School, Ann Arbor, 48109 MI.; Department of Psychiatry, University of Michigan Medical School, Ann Arbor, 48109 MI., Pritchett DL; Biology Department, Howard University, Washington, DC 20059., Jones KS; Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, 48109 MI kevjon@umich.edu.; Department of Pharmacology, University of Michigan Medical School, Ann Arbor, 48109 MI.
Jazyk:	angličtina
Zdroj:	ENeuro [eNeuro] 2023 Jul 24; Vol. 10 (7). Date of Electronic Publication: 2023 Jul 24 (Print Publication: 2023).
DOI:	10.1523/ENEURO.0167-23.2023
Abstrakt:	γ-Band oscillations (GBOs) are generated by fast-spiking interneurons (FSIs) and are critical for cognitive functions. Abnormalities in GBOs are frequently observed in schizophrenia and bipolar disorder and are strongly correlated with cognitive impairment. However, the underlying mechanisms are poorly understood. Studying GBOs in ex vivo preparations is challenging because of high energy demands and the need for continuous oxygen delivery to the tissue. As a result, GBOs are typically studied in brain tissue from very young animals or in experimental setups that maximize oxygen supply but compromise spatial resolution. Thus, there is a limited understanding of how GBOs interact within and between different brain structures and in brain tissue from mature animals. To address these limitations, we have developed a novel approach for studying GBOs in ex vivo hippocampal slices from mature animals, using 60-channel, perforated microelectrode arrays (pMEAs). pMEAs enhance oxygen delivery and increase spatial resolution in electrophysiological recordings, enabling comprehensive analyses of GBO synchronization within discrete brain structures. We found that transecting the Schaffer collaterals, a neural pathway within the hippocampus, impairs GBO coherence between CA1 and CA3 subfields. Furthermore, we validated our approach by studying GBO coherence in an Ank3 mutant mouse model exhibiting inhibitory synaptic dysfunction. We discovered that GBO coherence remains intact in the CA3 subfield of these mutant mice but is impaired within and between the CA1 subfield. Overall, our approach offers significant potential to characterize GBOs in ex vivo brain sections of animal models, enhancing our understanding of network dysfunction in psychiatric disorders. Competing Interests: The authors declare no competing financial interests. (Copyright © 2023 Rodríguez Díaz et al.)
Databáze:	MEDLINE
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