| Autor: |
Schoenmakers LLJ; Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands., Yewdall NA; Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands., Lu T; Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands., André AAM; Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands., Nelissen FHT; Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands., Spruijt E; Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands., Huck WTS; Institute for Molecules and Materials, Radboud University, 6525 AJ Nijmegen, The Netherlands. |
| Abstrakt: |
Biomolecular condensates are a promising platform for synthetic cell formation and constitute a potential missing link between the chemical and cellular stage of the origins of life. However, it has proven challenging to integrate complex reaction networks into biomolecular condensates, such as a cell-free in vitro transcription-translation (IVTT) system. Integrating IVTT into biomolecular condensates successfully is one precondition for condensation-based synthetic cell formation. Moreover, it would provide a proof of concept that biomolecular condensates are in principle compatible with the central dogma, one of the hallmarks of cellular life. Here, we have systemically investigated the compatibility of eight different (bio)molecular condensates with IVTT incorporation. Of these eight candidates, we have found that a green fluorescent protein-labeled, intrinsically disordered cationic protein (GFP-K 72 ) and single-stranded DNA (ssDNA) can form biomolecular condensates that are compatible with up to μM fluorescent protein expression. This shows that biomolecular condensates can indeed integrate complex reaction networks, confirming their use as synthetic cell platforms and hinting at a possible role in the origin of life. |
