| Autor: |
Jung HS; Laboratory of Single Molecule Biophysics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, United States.; Quantum Magnetic Imaging Team, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea., Cho KJ; Data Convergence Drug Research Center, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea., Joo S; Safety Measurement Institute, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea., Lee M; Safety Measurement Institute, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea., Kim MY; Safety Measurement Institute, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea., Kwon IH; Safety Measurement Institute, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea., Song NW; Quantum Magnetic Imaging Team, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea., Shim JH; Quantum Magnetic Imaging Team, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea.; Department of Applied Measurement Science, University of Science and Technology, Daejeon 34113, Republic of Korea., Neuman KC; Laboratory of Single Molecule Biophysics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, United States. |
| Abstrakt: |
Fluorescent nanodiamonds (FNDs) are versatile nanomaterials with promising properties. However, efficient functionalization of FNDs for biomedical applications remains challenging. In this study, we demonstrate mesoporous polydopamine (mPDA) encapsulation of FNDs. The mPDA shell is generated by sequential formation of micelles via self-assembly of Pluronic F127 (F127) with 1,3,5-trimethyl benzene (TMB) and composite micelles via oxidation and self-polymerization of dopamine hydrochloride (DA). The surface of the mPDA shell can be readily functionalized with thiol-terminated methoxy polyethylene glycol (mPEG-SH), hyperbranched polyglycerol (HPG), and d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS). The PEGylated FND@mPDA particles are efficiently taken up by, and employed as a fluorescent imaging probe for, HeLa cells. HPG-functionalized FND@mPDA is conjugated with an amino-terminated oligonucleotide to detect microRNA via hybridization. Finally, the increased surface area of the mPDA shell permits efficient loading of doxorubicin hydrochloride. Further modification with TPGS increases drug delivery efficiency, resulting in high toxicity to cancer cells. |
