| Autor: |
Dias FCR; Universidade Federal do Triângulo Mineiro - UFTM, Departamento de Biologia Estrutural, Uberaba, MG, Brasil.; Universidade Federal Rural de Pernambuco - UFRPE, Departamento de Veterinária, Recife, PE, Brasil., Cupertino MC; Universidade Federal de Viçosa - UFV, Departamento de Medicina e Nutrição, Laboratório de Métodos Epidemiológicos e Computacionais em Saúde, Viçosa, MG, Brasil.; Universidade Federal de Viçosa - UFV, Departamento de Biologia Geral, Viçosa, MG, Brasil., Silva PG; Universidade Federal de Viçosa - UFV, Departamento de Biologia Animal, Viçosa, MG, Brasil., Oliveira EL; Universidade Federal de Viçosa - UFV, Departamento de Biologia Geral, Viçosa, MG, Brasil., Ladeira LCM; Universidade Federal de Viçosa - UFV, Departamento de Biologia Geral, Viçosa, MG, Brasil., Matta SLP; Universidade Federal de Viçosa - UFV, Departamento de Biologia Geral, Viçosa, MG, Brasil., Otoni WC; Universidade Federal de Viçosa - UFV, Departamento de Biologia Vegetal, Viçosa, MG, Brasil., Gomes MLM; Universidade Federal do Triângulo Mineiro - UFTM, Departamento de Biologia Estrutural, Uberaba, MG, Brasil. |
| Abstrakt: |
Medicinal plant species are genetically engineered to obtain higher production of biomass and specific secondary metabolites, which can be used in the pharmaceutical industry. The aim of the present study was to evaluate the effect of Pfaffia glomerata (Spreng.) Pedersen tetraploid hydroalcoholic extract on the liver of adult Swiss mice. The extract was prepared from the plant roots and given to the animals by gavage, for 42 days. The experimental groups were treated with water (control), Pfaffia glomerata tetraploid hydroalcoholic extract (100, 200 and 400 mg/kg) and Pfaffia glomerata tetraploid hydroalcoholic extract discontinuously (200 mg/kg). The last group received the extract every 3 days, for 42 days. The oxidative status, mineral dynamics, and cell viability were analysed. The liver weight and the number of viable hepatocytes were reduced, despite the increased cell's number. Increased levels of malondialdehyde and nitric oxide, and changes in iron, copper, zinc, potassium, manganese and sodium levels were observed. aspartate aminotransferase levels were increased while alanine aminotransferase levels were decreased due to BGEt intake. Our results showed that BGEt induced alterations of oxidative stress biomarkers leading to liver injury, which was associated with a reduction in the number of hepatocytes. |
