[Relative survival and cell adhesion of multipotent mesenchymal cells from primary teeth pulp on the surface membranes and sponges produced from collagen].

Autor:	Badalyan VA; Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia., Vasilyev AV; Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia., Stepanyan ZM; Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia., Posessor AD; Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia., Bukharova TB; Borovsky Institute of Dentistry - Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia., Goldshtein DV; Bochkov Medical and Genetic Research Center, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Stomatologiia [Stomatologiia (Mosk)] 2023; Vol. 102 (3), pp. 5-10.
DOI:	10.17116/stomat20231020315
Abstrakt:	Objective: To study the behavior of SHED cell culture on different types of materials for the regeneration of periodontal tissues with different porosity. Material and Methods: Porous collagen material Fibro-Gide (Geitstlich Pharma AG, Switzerland), designed to increase the volume of the gum and the barrier collagen membrane Bio-Gide (Geitstlich Pharma AG, Switzerland) were studied in vitro on SHED cultures. As a control sample, a Spongostan sponge made of gelatin (Johnson & Johnson Medical, UK) with the most pronounced porosity and wettability was used. Acute cytotoxicity was determined using a screening method for assessing the number of living cells in a sample (MTT test). SHED cells were sown on the materials to study the attachment of cells to materials and their migration inside the samples. Before seeding, the cells were stained with vital fluorescent dye PKH26 (red fluorescent cell linker kit, Sigma, Germany) for further visualization. Results and Discussion: Using the MTT test it was shown that they do not have cytotoxic effects. At the same time by the 8th day of the experiment in the presence of Fibro-Gide and Bio-Gide the cells showed an increase in proliferative activity by 19% and 12%, respectively compared with the control group. The cells attached and spread out on the surface of the materials and migrated into the thickness of porous Fibro-Gide and Spongostan. Conclusion: The in vitro study showed that the most favorable material for SHED cell culture is the collagen material Fibro-Gide with sufficient porosity, elasticity and hydrophilicity. SHED cells attach to the collagen matrix and easily penetrate into the sample, filling the entire internal space, while the proliferative capacity of the cell culture increases.
Databáze:	MEDLINE
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