Topical formulations containing Trichilia catigua extract as therapeutic options for a genital and an acyclovir-resistant strain of herpes recurrent infection.

Autor: Ribelato EV; Laboratório de Virologia, Departamento de Microbiologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., Wouk J; Laboratório de Virologia, Departamento de Microbiologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., Celestino GG; Laboratório de Virologia, Departamento de Microbiologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., Rodrigues BCD; Laboratório de Virologia, Departamento de Microbiologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., Darido MLG; Laboratório de Virologia, Departamento de Microbiologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., Barboza MGL; Laboratório de Virologia, Departamento de Microbiologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., Botura TJ; Laboratório de Virologia, Departamento de Microbiologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., de Oliveira MC; Departamento de Histologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., de Andrade FG; Departamento de Histologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., Lonni AASG; Departamento de Ciências Farmacêuticas-Centro de Ciências da Saúde, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., de Mello JCP; Laboratório de Biologia Farmacêutica, Palafito, Departamento de Farmácia, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil., da Rocha SPD; Laboratório de Virologia, Departamento de Microbiologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil., Faccin-Galhardi LC; Laboratório de Virologia, Departamento de Microbiologia-Centro de Ciências Biológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil. lgalhardi@uel.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Sep; Vol. 54 (3), pp. 1501-1511. Date of Electronic Publication: 2023 Jun 20.
DOI: 10.1007/s42770-023-01027-w
Abstrakt: Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infect, respectively, 67% and 13% of the world population, most commonly causing mild symptoms, such as blisters/ulcers. However, severe conditions such as keratitis, encephalitis, and systemic infections may occur, generally associated with the patient's immunological condition. Although Acyclovir® (ACV) and its analogs are the reference drugs for herpetic infections, the number of ACV-resistant HSV infections is growing exponentially. Therefore, new natural products' bioactive compounds have been studied to develop novel effective anti-herpetics. Trichilia catigua is a plant widely used in traditional medicine, including the treatment of skin diseases and sexual infections. In our study, 16 extracts from the bark of T. catigua, obtained with different solvents and their combinations, were evaluated against HSV-1 AR and HSV-2, respectively, ACV resistance and genital strains in vitro. The extracts with the highest selectivity index were used to prepare new topical anti-herpetic formulations and confirmed in vivo. Two new topical formulations were suggested to treat cutaneous and genital herpetic recurrent lesions. The cytotoxicity and antiviral activity were tested using the MTT method. The cytotoxic (CC 50 ) and inhibitory (IC 50 ) concentrations of 50% and the selectivity index (SI: CC 50 /IC 50 ) were determined. Tc12, Tc13, and Tc16 were added to the formulations. Infected BALB/c mice were treated for 8 days, and the severity of the herpetic lesions was analyzed daily. All CEs showed a CC 50 value ranging from 143 to 400 µg/mL, except for Tc3 and Tc10. Tc12, Tc13, and Tc16 showed the best SI in the 0 h, virucidal, and adsorption inhibition assays. In the in vivo test against HSV-1 AR, the infected animals treated with creams were statistically different from the infected non-treated animals and similar to ACV-treated mice. In HSV-2-infected genitalia, similar effects were found for Tc13 and Tc16 gels. The present study demonstrated that extracts from the bark of T. catigua, traditionally used in folk medicine, are a valuable source of active compounds with anti-herpetic activity. The extracts showed a virucidal mechanism of action and prevented the initial stages of viral replication. The cutaneous and genital infections were strongly inhibited by the Tc12, Tc13, and Tc16 extracts. New topical therapeutic alternatives using Trichilia catigua extracts are suggested for patients infected with ACV-resistant strains of HSV.
(© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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