Humanized Mouse Model of HIV Infection.

Autor: Leontyev DS; Centre for Strategic Planning and Management of Biomedical Health Risks, Federal Medical-Biological Agency of Russia, Moscow, Russia. danila.leontyev@gmail.com., Glazkova DV; Centre for Strategic Planning and Management of Biomedical Health Risks, Federal Medical-Biological Agency of Russia, Moscow, Russia., Bezborodova OA; P. A. Hertsen Moscow Oncology Research Institute - Affiliated Branch of National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia., Tsyganova GM; Centre for Strategic Planning and Management of Biomedical Health Risks, Federal Medical-Biological Agency of Russia, Moscow, Russia., Urusov FA; Centre for Strategic Planning and Management of Biomedical Health Risks, Federal Medical-Biological Agency of Russia, Moscow, Russia.; N. F. Izmerov Research Institute of Occupational Health, Moscow, Russia., Pankratov AA; P. A. Hertsen Moscow Oncology Research Institute - Affiliated Branch of National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia., Shipulin GA; Centre for Strategic Planning and Management of Biomedical Health Risks, Federal Medical-Biological Agency of Russia, Moscow, Russia., Bogoslovskaya EV; Centre for Strategic Planning and Management of Biomedical Health Risks, Federal Medical-Biological Agency of Russia, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2023 May; Vol. 175 (1), pp. 63-66. Date of Electronic Publication: 2023 Jun 20.
DOI: 10.1007/s10517-023-05812-3
Abstrakt: The development of new drugs for the treatment of HIV infection requires testing of their efficacy in a relevant animal model, such as humanized mice, which, unfortunately, are not yet available in Russia. In the present study, we have developed conditions for the humanization of immunodeficient NSG mice with human hematopoietic stem cells. Humanized animals generated during the study showed a high degree of chimerism and harbored repopulation of the entire range of human lymphocytes required for HIV replication in the blood and organs. Inoculation of these mice with HIV-1 virus led to stable viremia, which was confirmed by the presence of viral RNA in blood plasma throughout the entire period of observation and proviral DNA in the organs of animals 4 weeks after HIV infection.
(© 2023. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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