Shifts in carbon partitioning by photosynthetic activity increase terpenoid synthesis in glandular trichomes.

Autor: Saadat NP; Institute of Theoretical and Quantitative Biology, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.; Cluster of Excellence on Plant Sciences, Heinrich Heine University Düsseldorf, Universitätsstraße 1, 40225, Düsseldorf, Germany., van Aalst M; Institute of Theoretical and Quantitative Biology, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany., Brand A; Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle, Germany., Ebenhöh O; Institute of Theoretical and Quantitative Biology, Heinrich Heine University Düsseldorf, 40225, Düsseldorf, Germany.; Cluster of Excellence on Plant Sciences, Heinrich Heine University Düsseldorf, Universitätsstraße 1, 40225, Düsseldorf, Germany., Tissier A; Cluster of Excellence on Plant Sciences, Heinrich Heine University Düsseldorf, Universitätsstraße 1, 40225, Düsseldorf, Germany., Matuszyńska AB; Cluster of Excellence on Plant Sciences, Heinrich Heine University Düsseldorf, Universitätsstraße 1, 40225, Düsseldorf, Germany.; Computational Life Science, Department of Biology, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2023 Sep; Vol. 115 (6), pp. 1716-1728. Date of Electronic Publication: 2023 Jul 04.
DOI: 10.1111/tpj.16352
Abstrakt: Several commercially important secondary metabolites are produced and accumulated in high amounts by glandular trichomes, giving the prospect of using them as metabolic cell factories. Due to extremely high metabolic fluxes through glandular trichomes, previous research focused on how such flows are achieved. The question regarding their bioenergetics became even more interesting with the discovery of photosynthetic activity in some glandular trichomes. Despite recent advances, how primary metabolism contributes to the high metabolic fluxes in glandular trichomes is still not fully elucidated. Using computational methods and available multi-omics data, we first developed a quantitative framework to investigate the possible role of photosynthetic energy supply in terpenoid production and next tested experimentally the simulation-driven hypothesis. With this work, we provide the first reconstruction of specialised metabolism in Type-VI photosynthetic glandular trichomes of Solanum lycopersicum. Our model predicted that increasing light intensities results in a shift of carbon partitioning from catabolic to anabolic reactions driven by the energy availability of the cell. Moreover, we show the benefit of shifting between isoprenoid pathways under different light regimes, leading to a production of different classes of terpenes. Our computational predictions were confirmed in vivo, demonstrating a significant increase in production of monoterpenoids while the sesquiterpenes remained unchanged under higher light intensities. The outcomes of this research provide quantitative measures to assess the beneficial role of chloroplast in glandular trichomes for enhanced production of secondary metabolites and can guide the design of new experiments that aim at modulating terpenoid production.
(© 2023 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE