CMA/DAPI Banding of Plant Chromosomes.

Autor: Barros E Silva AE; Laboratório de Citogenética Vegetal, Departamento de Biociências, Centro de Ciências Agrárias, Universidade Federal da Paraíba, Areia, Paraíba, Brazil., Guerra M; Laboratório de Citogenética e Evolução Vegetal, Departamento de Botânica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2672, pp. 215-224.
DOI: 10.1007/978-1-0716-3226-0_12
Abstrakt: Chromosome banding based on base-specific fluorochromes, mainly double staining with chromomycin A 3 (CMA) and 4'-6-diamidino-2-phenylindole (DAPI), has been widely used since the 1970s. This technique allows the differential staining of distinct types of heterochromatin. Afterward, the fluorochromes can be easily removed and leave the preparation ready for sequential procedures such as FISH or immunodetection. Interpretations of similar bands obtained with different techniques, however, merit certain caution. Here we present a detailed protocol for CMA/DAPI staining optimized for plant cytogenetics and call attention to the most common sources of misinterpretation of DAPI bands.
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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