Preparation of Mitotic Chromosomes with the Squash Technique.
| Autor: | Maravilla AJ; Jardín Botánico, ICBiBE, Universitat de València, València, Spain., Rosato M; Dpt. Genética, Fisiología y Microbiología (UD Genética), Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Madrid, Spain. crosato@ucm.es., Rosselló JA; Jardín Botánico, ICBiBE, Universitat de València, València, Spain. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2672, pp. 141-149. |
| DOI: | 10.1007/978-1-0716-3226-0_7 |
| Abstrakt: | Plant chromosomes are usually obtained from meristematic tissue of active root tips through the conventional squash method. Nevertheless, cytogenetic work usually implies a great effort and some modifications of standard procedures need to be evaluated. In this chapter, we describe our outline for handling chromosomes using the squash method. By using these protocols, high-quality chromosome spreads are obtained, which allow chromosome counting, building karyotypes, and assessing chromosomal landmarks, and enable genome mapping by fluorochrome banding and in situ hybridization techniques. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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