Opine dehydrogenases, an underexplored enzyme family for the enzymatic synthesis of chiral amines.

Autor: Telek A; Department of Applied Biotechnology, Budapest University of Technology and Economics, Budapest, Hungary.; Servier Research Institute of Medicinal Chemistry, Budapest, Hungary., Molnár Z; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary.; Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Budapest, Hungary., Vértessy BG; Department of Applied Biotechnology, Budapest University of Technology and Economics, Budapest, Hungary.; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary., Tasnádi G; Servier Research Institute of Medicinal Chemistry, Budapest, Hungary.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2023 Oct; Vol. 120 (10), pp. 2793-2808. Date of Electronic Publication: 2023 Jun 19.
DOI: 10.1002/bit.28469
Abstrakt: Opines and opine-type chemicals are valuable natural products with diverse biochemical roles, and potential synthetic building blocks of bioactive compounds. Their synthesis involves reductive amination of ketoacids with amino acids. This transformation has high synthetic potential in producing enantiopure secondary amines. Nature has evolved opine dehydrogenases for this chemistry. To date, only one enzyme has been used as biocatalyst, however, analysis of the available sequence space suggests more enzymes to be exploited in synthetic organic chemistry. This review summarizes the current knowledge of this underexplored enzyme class, highlights key molecular, structural, and catalytic features with the aim to provide a comprehensive general description of opine dehydrogenases, thereby supporting future enzyme discovery and protein engineering studies.
(© 2023 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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