The human Y and inactive X chromosomes similarly modulate autosomal gene expression.

Autor:	San Roman AK; Whitehead Institute; Cambridge, MA 02142, USA., Skaletsky H; Whitehead Institute; Cambridge, MA 02142, USA.; Howard Hughes Medical Institute, Whitehead Institute; Cambridge, MA 02142, USA., Godfrey AK; Whitehead Institute; Cambridge, MA 02142, USA.; Department of Biology, Massachusetts Institute of Technology; Cambridge, MA 02139, USA., Bokil NV; Whitehead Institute; Cambridge, MA 02142, USA.; Department of Biology, Massachusetts Institute of Technology; Cambridge, MA 02139, USA., Teitz L; Whitehead Institute; Cambridge, MA 02142, USA.; Department of Biology, Massachusetts Institute of Technology; Cambridge, MA 02139, USA., Singh I; Whitehead Institute; Cambridge, MA 02142, USA.; Harvard Medical School, Boston, MA 02115, USA., Blanton LV; Whitehead Institute; Cambridge, MA 02142, USA., Bellott DW; Whitehead Institute; Cambridge, MA 02142, USA., Pyntikova T; Whitehead Institute; Cambridge, MA 02142, USA., Lange J; Whitehead Institute; Cambridge, MA 02142, USA.; Department of Biology, Massachusetts Institute of Technology; Cambridge, MA 02139, USA., Koutseva N; Whitehead Institute; Cambridge, MA 02142, USA., Hughes JF; Whitehead Institute; Cambridge, MA 02142, USA., Brown L; Whitehead Institute; Cambridge, MA 02142, USA.; Howard Hughes Medical Institute, Whitehead Institute; Cambridge, MA 02142, USA., Phou S; Whitehead Institute; Cambridge, MA 02142, USA.; Howard Hughes Medical Institute, Whitehead Institute; Cambridge, MA 02142, USA., Buscetta A; Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda; MD 20892, USA., Kruszka P; Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda; MD 20892, USA., Banks N; Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda; MD 20892, USA.; Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health; Bethesda, MD 20892 USA., Dutra A; Cytogenetics and Microscopy Core, National Human Genome Research Institute, National Institutes of Health; Bethesda, MD 20892 USA., Pak E; Cytogenetics and Microscopy Core, National Human Genome Research Institute, National Institutes of Health; Bethesda, MD 20892 USA., Lasutschinkow PC; Focus Foundation, Davidsonville, MD 21035, USA., Keen C; Focus Foundation, Davidsonville, MD 21035, USA., Davis SM; Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA., Lin AE; Medical Genetics, Massachusetts General for Children, Boston, MA 02114, USA; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA., Tartaglia NR; Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA.; Developmental Pediatrics, eXtraOrdinarY Kids Program, Children's Hospital Colorado, Aurora, CO 80011, USA., Samango-Sprouse C; Focus Foundation, Davidsonville, MD 21035, USA.; Department of Pediatrics, George Washington University, Washington, DC 20052, USA; Department of Human and Molecular Genetics, Florida International University, Miami, FL 33199, USA., Muenke M; Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda; MD 20892, USA., Page DC; Whitehead Institute; Cambridge, MA 02142, USA.; Department of Biology, Massachusetts Institute of Technology; Cambridge, MA 02139, USA.; Howard Hughes Medical Institute, Whitehead Institute; Cambridge, MA 02142, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Jun 07. Date of Electronic Publication: 2023 Jun 07.
DOI:	10.1101/2023.06.05.543763
Abstrakt:	Somatic cells of human males and females have 45 chromosomes in common, including the "active" X chromosome. In males the 46 th chromosome is a Y; in females it is an "inactive" X (Xi). Through linear modeling of autosomal gene expression in cells from individuals with zero to three Xi and zero to four Y chromosomes, we found that Xi and Y impact autosomal expression broadly and with remarkably similar effects. Studying sex-chromosome structural anomalies, promoters of Xi- and Y-responsive genes, and CRISPR inhibition, we traced part of this shared effect to homologous transcription factors - ZFX and ZFY - encoded by Chr X and Y. This demonstrates sex-shared mechanisms by which Xi and Y modulate autosomal expression. Combined with earlier analyses of sex-linked gene expression, our studies show that 21% of all genes expressed in lymphoblastoid cells or fibroblasts change expression significantly in response to Xi or Y chromosomes. Competing Interests: Declaration of interests: The authors declare no competing interests.
Databáze:	MEDLINE
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