Transcriptomics reveal the genetic coordination of early defense to Armillaria root rot (ARR) in Prunus spp.

Autor:	Cai L; Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States., Adelberg J; Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States., Naylor-Adelberg J; Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States., Schnabel G; Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States., Calle A; Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States., Li Z; Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States., Reighard G; Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States., Gasic K; Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States., Saski CA; Department of Plant and Environmental Sciences, Clemson University, Clemson, SC, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2023 Jun 02; Vol. 14, pp. 1181153. Date of Electronic Publication: 2023 Jun 02 (Print Publication: 2023).
DOI:	10.3389/fpls.2023.1181153
Abstrakt:	Armillaria root rot (ARR) poses a significant threat to the long-term productivity of stone-fruit and nut crops in the predominant production area of the United States. To mitigate this issue, the development of ARR-resistant and horticulturally-acceptable rootstocks is a crucial step towards the maintenance of production sustainability. To date, genetic resistance to ARR has been found in exotic plum germplasm and a peach/plum hybrid rootstock, 'MP-29'. However, the widely-used peach rootstock Guardian® is susceptible to the pathogen. To understand the molecular defense mechanisms involved in ARR resistance in Prunus rootstocks, transcriptomic analyses of one susceptible and two resistant Prunus spp. were performed using two causal agents of ARR, including Armillaria mellea and Desarmillaria tabescens . The results of in vitro co-culture experiments revealed that the two resistant genotypes showed different temporal response dynamics and fungus-specific responses, as seen in the genetic response. Gene expression analysis over time indicated an enrichment of defense-related ontologies, including glucosyltransferase activity, monooxygenase activity, glutathione transferase activity, and peroxidase activity. Differential gene expression and co-expression network analysis highlighted key hub genes involved in the sensing and enzymatic degradation of chitin, GSTs, oxidoreductases, transcription factors, and biochemical pathways likely involved in Armillaria resistance. These data provide valuable resources for the improvement of ARR resistance in Prunus rootstocks through breeding. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Cai, Adelberg, Naylor-Adelberg, Schnabel, Calle, Li, Reighard, Gasic and Saski.)
Databáze:	MEDLINE
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