Boar sperm motility is modulated by CCK at a low concentration of bicarbonate under capacitation conditions.

Autor: Moros-Nicolás C; Departamento de Biología Celular e Histología, Facultad de Medicina, Campus de Ciencias de la Salud, Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain.; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de la Salud, Murcia, Spain., López-Úbeda R; Departamento de Biología Celular e Histología, Facultad de Medicina, Campus de Ciencias de la Salud, Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain.; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de la Salud, Murcia, Spain., Luongo C; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de la Salud, Murcia, Spain.; Departamento de Fisiología, Facultad de Veterinaria, Campus de Espinardo, Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain., Sòria-Monzó P; Departamento de Biología Celular e Histología, Facultad de Medicina, Campus de Ciencias de la Salud, Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain.; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de la Salud, Murcia, Spain., Abril-Sánchez S; Departamento de Fisiología, Facultad de Veterinaria, Campus de Espinardo, Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain., Grudzinska P; Departamento de Fisiología, Facultad de Veterinaria, Campus de Espinardo, Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain., Avilés M; Departamento de Biología Celular e Histología, Facultad de Medicina, Campus de Ciencias de la Salud, Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain.; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de la Salud, Murcia, Spain., Izquierdo-Rico MJ; Departamento de Biología Celular e Histología, Facultad de Medicina, Campus de Ciencias de la Salud, Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain.; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de la Salud, Murcia, Spain., García-Vázquez FA; Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB), Campus de Ciencias de la Salud, Murcia, Spain.; Departamento de Fisiología, Facultad de Veterinaria, Campus de Espinardo, Campus Mare Nostrum (CMN), Universidad de Murcia, Murcia, Spain.
Jazyk: angličtina
Zdroj: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2023 Aug; Vol. 58 (8), pp. 1164-1171. Date of Electronic Publication: 2023 Jun 23.
DOI: 10.1111/rda.14412
Abstrakt: In a previous study, our group detected the cholecystokinin (CCK) protein in the porcine oviduct. This fact, together with the involvement of CCK in the regulation of sperm protein tyrosine phosphorylation by the modulation of HCO 3 - uptake (in mice and humans) suggests a role for CCK during sperm capacitation. Therefore, on the one hand, the expression of CCK receptors (CCK1R and CCK2R) on boar testes has been investigated and probed; on the other hand, boar spermatozoa (from seminal doses of 1-day and 5-day storage) were exposed to different concentrations of CCK (0-control, 25 or 50 μM) in a medium supporting capacitation supplemented with 0, 5 or 25 mmol/L of HCO 3 - for 1 h at 38.5°C. Sperm motion (total and progressive motility), kinetic parameters, viability, acrosome status, and mitochondrial activity were determined. No differences between groups (0, 25 or 50 μM of CCK) were observed when HCO 3 - was absent in the media (p > .05). However, the results showed that when the media was supplemented with 5 mmol/L HCO 3 - in 1-day seminal dose storage, the linearity index (LIN, %), straightness index (STR, %) and oscillation index (WOB, %) (sperm kinetics parameters) increased in the presence of CCK regardless the concentration (p < .05). Nevertheless, CCK in sperm from 5-day storage only increased the WOB parameter in comparison to the control (p < .05). Furthermore, the average amplitude of the lateral displacement of the sperm head (ALH, μm) and curvilinear velocity (VCL, μm/s) decreased when CCK was present, depending on its concentration and sperm aging (1-day vs. 5-days) (p < .05). In the case of the media supporting capacitation supplemented with 25 mmol/L HCO 3 - , any differences were observed except for sperm viability in the 5-day seminal doses, which increased in the 50 μM-CCK group compared to the control (p < .05). In conclusion, these data suggest an implication of CCK protein during sperm capacitation under low bicarbonate concentration increasing the sperm linear trajectory.
(© 2023 The Authors. Reproduction in Domestic Animals published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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