Exposure to pyrogallol impacts the hemato-biochemical endpoints in catfish (Clarias gariepinus).

Autor: Hamed M; Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), Assiut, 71524, Egypt., Martyniuk CJ; Center for Environmental and Human Toxicology, Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32611, USA., Said REM; Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), Assiut, 71524, Egypt., Soliman HAM; Department of Zoology, Faculty of Science, Sohag University, Sohag, 8562, Egypt., Badrey AEA; Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), Assiut, 71524, Egypt., Hassan EA; Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, 71516, Egypt., Abdelhamid HN; Advanced Multifunctional Materials Laboratory, Department of Chemistry, Assuit University, Assuit, 71515, Egypt; Nanotechnology Research Centre (NTRC), The British University in Egypt, El-Shorouk City, Suez Desert Road, P.O. Box 43, Cairo 11837, Egypt., Osman AGM; Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), Assiut, 71524, Egypt., Sayed AEH; Department of Zoology, Faculty of Science, Assiut University, Assiut, 71516, Egypt; Molecular Biology Research & Studies Institute, Assiut University, 71516 Assiut, Egypt. Electronic address: alaasayed@aun.edu.eg.
Jazyk: angličtina
Zdroj: Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2023 Sep 15; Vol. 333, pp. 122074. Date of Electronic Publication: 2023 Jun 16.
DOI: 10.1016/j.envpol.2023.122074
Abstrakt: Pyrogallol is widely used in several industrial applications and can subsequently contaminate aquatic ecosystems. Here, we report for the first time the presence of pyrogallol in wastewater in Egypt. Currently, there is a complete lack of toxicity and carcinogenicity data for pyrogallol exposure in fish. To address this gap, both acute and sub-acute toxicity experiments were conducted to determine the toxicity of pyrogallol in catfish (Clarias gariepinus). Behavioral and morphological endpoints were evaluated, in addition to blood hematological endpoints, biochemical indices, electrolyte balance, and the erythron profile (poikilocytosis and nuclear abnormalities). In the acute toxicity assay, it was determined that the 96 h median-lethal concentration (96 h-LC 50 ) of pyrogallol for catfish was 40 mg/L. In sub-acute toxicity experiment, fish divided into four groups; Group 1 was the control group. Group 2 was exposed to 1 mg/L of pyrogallol, Group 3 was exposed to 5 mg/L of pyrogallol, and Group 4 was exposed to 10 mg/L of pyrogallol. Fish showed morphological changes such as erosion of the dorsal and caudal fins, skin ulcers, and discoloration following exposure to pyrogallol for 96 h. Exposure to 1, 5, or 10 mg/L pyrogallol caused a significant decrease in hematological indices, including red blood cells (RBCs), hemoglobin, hematocrit, white blood cells (WBC), thrombocytes, and large and small lymphocytes in a dose-dependent manner. Several biochemical parameters (creatinine, uric acid, liver enzymes, lactate dehydrogenase, and glucose) were altered in a concentration dependent manner with short term exposures to pyrogallol. Pyrogallol exposure also caused a significant concentration-dependent rise in the percentage of poikilocytosis and nuclear abnormalities of RBCs in catfish. In conclusion, our data suggest that pyrogallol should be considered further in environmental risk assessments of aquatic species.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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