In vitro ovicidal effect of p-coumaric acid from Acacia bilimekii aerial parts against Haemonchus contortus.

Autor: Cortes-Morales JA; Laboratorio de Fitoquímica y Productos Naturales del Centro de Investigación en Biodiversidad y Conservación, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos, Mexico., Zamilpa A; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1. Col. Centro., C.P. 62790 Xochitepec, Morelos, Mexico. Electronic address: azamilpa_2000@yahoo.com.mx., Salinas-Sánchez DO; Laboratorio de Fitoquímica y Productos Naturales del Centro de Investigación en Biodiversidad y Conservación, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos, Mexico., González-Cortazar M; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1. Col. Centro., C.P. 62790 Xochitepec, Morelos, Mexico., Tapia-Maruri D; Departamento de Biotecnología, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, PO Box 24, Yautepec, Morelos C.P. 62730, Mexico., Gives PM; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID SAI-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534 / Col. Progreso., C.P. 62574 Jiutepec, Morelos / A.P. 206-CIVAC, Mexico., Rivas-González JM; Escuela de Estudios Superiores del Jicarero. Carretera Galeana, Tequesquitengo s/n, Comunidad El Jicarero, Jojutla, Morelos, Mexico., Olmedo-Juárez A; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID SAI-INIFAP), Carretera Federal Cuernavaca-Cuautla No. 8534 / Col. Progreso., C.P. 62574 Jiutepec, Morelos / A.P. 206-CIVAC, Mexico. Electronic address: olmedo.agustin@inifap.gob.mx.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2023 Aug; Vol. 320, pp. 109971. Date of Electronic Publication: 2023 Jun 08.
DOI: 10.1016/j.vetpar.2023.109971
Abstrakt: Acacia bilimekii is a plant with a high content of protein, fibre, and condensed tannins, making it an excellent feed for small ruminants with anthelmintic potential. This study aimed to evaluate the ovicidal activity of a hydroalcoholic extract (Ab-HA) and fractions from A. bilimekii aerial parts on Haemonchus contortus. The ovicidal activity of the Ab-HA extract and its fractions obtained by chromatographic fractionation were evaluated through the egg hatching inhibition (EHI) test. The results showed that the Ab-HA extract had 91% EHI at 20,000 µg/mL with a mean effective concentration (EC 50 ) of 9260 µg/mL. After liquid-liquid fractionation of Ab-HA extract, the aqueous fraction (Ab-Aq) did not show ovicidal activity, whereas the organic fraction (Ab-EtOAc) showed a better EHI than the Ab-HA extract (98.9% at 2500 µg/mL). Then, the chemical fractionation of Ab-EtOAc allowed obtaining six bioactive fractions (AbR12-17) with an EHI greater than 90% at 1500 µg/mL. The best treatment was AbR15 (98.7% EHI at 750 µg/mL). Chemical analysis by HPLC-PDA of AbR15 indicated the presence of p-coumaric acid and the flavone luteolin as major compounds. Additionally, the commercial p-coumaric acid standard was evaluated in the EHI assay and showed an EHI of 97% at 62.5 µg/mL. Meanwhile, the confocal laser scanning microscopy analysis demonstrated a colocalization effect between p-coumaric acid and the H. contortus embryonated eggs. These results indicate that due to their major chemical compounds (including p-coumaric acid), the aerial parts of the plant A. bilimekii, could be considered as natural potential tool for controlling haemonchosis in small ruminants.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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