Human cells experience a Zn 2+ pulse in early G1.

Autor:	Rakshit A; Department of Biochemistry and BioFrontiers Institute, 3415 Colorado Avenue, University of Colorado Boulder, Boulder, CO 80303, USA., Holtzen SE; Department of Molecular Cellular Developmental Biology and BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80309, USA., Lo MN; Department of Biochemistry and BioFrontiers Institute, 3415 Colorado Avenue, University of Colorado Boulder, Boulder, CO 80303, USA., Conway KA; Department of Biochemistry and BioFrontiers Institute, 3415 Colorado Avenue, University of Colorado Boulder, Boulder, CO 80303, USA., Palmer AE; Department of Biochemistry and BioFrontiers Institute, 3415 Colorado Avenue, University of Colorado Boulder, Boulder, CO 80303, USA. Electronic address: amy.palmer@colorado.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2023 Jun 27; Vol. 42 (6), pp. 112656. Date of Electronic Publication: 2023 Jun 17.
DOI:	10.1016/j.celrep.2023.112656
Abstrakt:	Zinc is an essential micronutrient required for all domains of life. Cells maintain zinc homeostasis using a network of transporters, buffers, and transcription factors. Zinc is required for mammalian cell proliferation, and zinc homeostasis is remodeled during the cell cycle, but whether labile zinc changes in naturally cycling cells has not been established. We use genetically encoded fluorescent reporters, long-term time-lapse imaging, and computational tools to track labile zinc over the cell cycle in response to changes in growth media zinc and knockdown of the zinc-regulatory transcription factor MTF-1. Cells experience a pulse of labile zinc in early G1, whose magnitude varies with zinc in growth media. Knockdown of MTF-1 increases labile zinc and the zinc pulse. Our results suggest that cells need a minimum zinc pulse to proliferate and that if labile zinc levels are too high, cells pause proliferation until labile cellular zinc is lowered. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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