Culture Media Supplemented With 10% Equine Serum Provided Chondroprotection in an In Vitro Co-Culture of Cartilage and Synovial Membrane.

Autor: Velloso Alvarez A; Department of clinical sciences, Auburn University, Auburn, AL; Universidad CEU-Cardenal Herrera, CEU Universities, Alfara del Patriarca, Valencia, Spain., Wooldridge AA; Department of clinical sciences, Auburn University, Auburn, AL., Fuller J; Department of clinical sciences, Auburn University, Auburn, AL., Shrader SM; Department of pathobiology, Auburn University, Auburn, AL., Mansour M; Department of anatomy, physiology and pharmacology, Auburn University, Auburn, AL., Boone LH; Department of clinical sciences, Auburn University, Auburn, AL. Electronic address: lhb0021@auburn.edu.
Jazyk: angličtina
Zdroj: Journal of equine veterinary science [J Equine Vet Sci] 2023 Sep; Vol. 128, pp. 104865. Date of Electronic Publication: 2023 Jun 15.
DOI: 10.1016/j.jevs.2023.104865
Abstrakt: No studies have evaluated the effect of culture in serum-free media (SF) vs. media supplemented with equine serum (ES) on co-culture of synovial membrane and cartilage tissue explants. The study objective was to evaluate the effects of equine serum supplementation on induced production of inflammatory and catabolic mediators from articular cartilage and synovial explants while in co-culture. Articular cartilage and synovial membrane explants were harvested from femoropatellar joints of five adult horses. Cartilage and synovial explants were harvested from the stifle of five horses, placed in co-culture, stimulated with IL-1β (10 ng/ml) and maintained in culture for 3, 6 and 9 days in 10% ES or SF. At each time point, media was harvested for analysis of cellular viability (Lactate dehydrogenase) and elution of glycosaminoglycans (Dimethylene Blue Binding Assay). Tissue explants were harvested for histopathologic and gene expression analyses. No differences in cell viability were observed between SF and ES groups. SF culture produced an upregulation of TNF-α in synovial membrane and ADAMTS-4 and five in articular cartilage at 9 days of culture. ES produced an upregulation of aggrecan expression in cartilage at 9 days of culture. No differences in tissue viability were found between culture media, but SF media produced a higher glycosaminoglycan concentration in media at 3 days of culture. The addition of 10% ES produced a slight chondroprotective effect in an inflamed co-culture system. This effect should be considered when designing studies evaluating treatment of serum or plasma-based orthobiologic studies in vitro.
Competing Interests: Declaration of Competing Interest This study has been funded by Auburn University Intramural Grants Program Faculty Research Initiation Grant to Lindsey H. Boone. None of the authors have any conflict of interest to declare.
(Published by Elsevier Inc.)
Databáze: MEDLINE
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