Super-resolution vibrational imaging based on photoswitchable Raman probe.

Autor: Shou J; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan., Komazawa A; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan., Wachi Y; Department of Electrical and Electronic Engineering, The University of Tokyo, Tokyo 113-8656, Japan., Kawatani M; Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.; Department of Life Science and Technology, Tokyo Institute of Technology, Kanagawa 226-8501, Japan., Fujioka H; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan., Spratt SJ; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan., Mizuguchi T; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan., Oguchi K; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan., Akaboshi H; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan., Obata F; Laboratory for Nutritional Biology, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo 650-0047, Japan., Tachibana R; Department of Chemistry, University of Basel, 4056 Basel, Switzerland., Yasunaga S; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan.; Laboratory for Integrated Micro Mechatronic Systems, National Center for Scientific Research, The University of Tokyo, Tokyo 153-8505, Japan., Mita Y; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan.; Laboratory for Integrated Micro Mechatronic Systems, National Center for Scientific Research, The University of Tokyo, Tokyo 153-8505, Japan., Misawa Y; Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.; Department of Life Science and Technology, Tokyo Institute of Technology, Kanagawa 226-8501, Japan., Kojima R; Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan., Urano Y; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.; Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan., Kamiya M; Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.; Department of Life Science and Technology, Tokyo Institute of Technology, Kanagawa 226-8501, Japan.; Living Systems Materialogy (LiSM) Research Group, International Research Frontiers Initiative (IRFI), Tokyo Institute of Technology, Kanagawa 226-8501 Japan., Ozeki Y; Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2023 Jun 16; Vol. 9 (24), pp. eade9118. Date of Electronic Publication: 2023 Jun 16.
DOI: 10.1126/sciadv.ade9118
Abstrakt: Super-resolution vibrational microscopy is promising to increase the degree of multiplexing of nanometer-scale biological imaging because of the narrower spectral linewidth of molecular vibration compared to fluorescence. However, current techniques of super-resolution vibrational microscopy suffer from various limitations including the need for cell fixation, high power loading, or complicated detection schemes. Here, we present reversible saturable optical Raman transitions (RESORT) microscopy, which overcomes these limitations by using photoswitchable stimulated Raman scattering (SRS). We first describe a bright photoswitchable Raman probe (DAE620) and validate its signal activation and depletion characteristics when exposed to low-power (microwatt level) continuous-wave laser light. By harnessing the SRS signal depletion of DAE620 through a donut-shaped beam, we demonstrate super-resolution vibrational imaging of mammalian cells with excellent chemical specificity and spatial resolution beyond the optical diffraction limit. Our results indicate RESORT microscopy to be an effective tool with high potential for multiplexed super-resolution imaging of live cells.
Databáze: MEDLINE