Experimentally exposed toxic effects of long-term exposure to environmentally relevant concentrations of CIP in males and females of the silver catfish Rhamdia quelen.

Autor: Carvalho LÂSJ; Ecology and Conservation Program Post-Graduation, Federal University of Paraná, PO Box 19031, 81531-980, Curitiba, PR, Brazil., Oya-Silva LF; Department of Genetics, Federal University of Paraná, PO Box 19071, 81530-980, Curitiba, PR, Brazil., Perussolo MC; Pelé Pequeno Principe Research Institute, 80.250-200, Curitiba, PR, Brazil; Department of Pharmacology, Federal University of Paraná, PO Box 19031, 81530-980, Curitiba, PR, Brazil., Oliveira Guaita G; Department of Pharmacology, Federal University of Paraná, PO Box 19031, 81530-980, Curitiba, PR, Brazil., Moreira Brito JC; Ezequiel Dias Foundation, 30510-010, Belo Horizonte, MG, Brazil., Evans AA; Department of Pharmacology, Federal University of Paraná, PO Box 19031, 81530-980, Curitiba, PR, Brazil; School of Medicine, Pequeno Principe Faculty, 80.230-020, Curitiba, PR, Brazil., Prodocimo MM; Department of Cell Biology, Federal University of Paraná, PO Box 19031, 81530-980, Curitiba, PR, Brazil., Cestari MM; Department of Genetics, Federal University of Paraná, PO Box 19071, 81530-980, Curitiba, PR, Brazil., Braga TT; Department of Pathology, Federal University of Paraná, Curitiba, Brazil; Biosciences and Biotechnology Graduation Program, Instituto Carlos Chagas (ICC), Fiocruz, Curitiba, PR, Brazil., Silva de Assis HC; Ecology and Conservation Program Post-Graduation, Federal University of Paraná, PO Box 19031, 81531-980, Curitiba, PR, Brazil; Department of Pharmacology, Federal University of Paraná, PO Box 19031, 81530-980, Curitiba, PR, Brazil. Electronic address: helassis@ufpr.br.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2023 Sep; Vol. 336, pp. 139216. Date of Electronic Publication: 2023 Jun 13.
DOI: 10.1016/j.chemosphere.2023.139216
Abstrakt: Ciprofloxacin (CIP) is an antibiotic commonly used in human and veterinary medicine. It is present in the aquatic environment, but we still know very little about its effect on non-targeted organisms. This study aimed to evaluate the effects of long-term exposure to environmental CIP concentrations (1, 10, and 100 μg.L -1 ) in males and females of Rhamdia quelen. After 28 days of exposure, we collected the blood for the analysis of hematological and genotoxic biomarkers. Additionally, we measured 17 β-estradiol and 11 keto-testosterone levels. After the euthanasia, we collected the brain and the hypothalamus to analyze acetylcholinesterase (AChE) activity and neurotransmitters, respectively. The liver and gonads were assessed for biochemical, genotoxic, and histopathological biomarkers. At 100 μg.L -1 CIP, we observed genotoxicity in the blood, nuclear morphological changes, apoptosis, leukopenia, and a reduction of AChE in the brain. In the liver was observed oxidative stress and apoptosis. At 10 μg.L -1 CIP, leukopenia, morphological changes, and apoptosis were presented in the blood and a reduction of AChE in the brain. Apoptosis, leukocyte infiltration, steatosis, and necrosis occurred in the liver. Even at the lowest concentration (1 μg.L -1 ), adverse effects such as erythrocyte and liver genotoxicity, hepatocyte apoptosis, oxidative stress, and a decrease in somatic indexes were observed. The results showed the importance of monitoring CIP concentrations in the aquatic environment that cause sublethal effects on fish.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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