Labeling PIEZO2 activity in the peripheral nervous system.

Autor:	Villarino NW; Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, The Scripps Research Institute, La Jolla, CA 92037, USA., Hamed YMF; Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA; Graduate Program in Development, Disease Models, and Therapeutics, Baylor College of Medicine, Houston, TX 77030., Ghosh B; Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA., Dubin AE; Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA., Lewis AH; Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA., Odem MA; Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA., Loud MC; Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, The Scripps Research Institute, La Jolla, CA 92037, USA., Wang Y; Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA., Servin-Vences MR; Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA., Patapoutian A; Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, The Scripps Research Institute, La Jolla, CA 92037, USA. Electronic address: ardem@scripps.edu., Marshall KL; Department of Neuroscience, Dorris Neuroscience Center, The Scripps Research Institute, La Jolla, CA 92037, USA; Howard Hughes Medical Institute, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA; Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, TX 77030, USA. Electronic address: kara.marshall@bcm.edu.
Jazyk:	angličtina
Zdroj:	Neuron [Neuron] 2023 Aug 16; Vol. 111 (16), pp. 2488-2501.e8. Date of Electronic Publication: 2023 Jun 14.
DOI:	10.1016/j.neuron.2023.05.015
Abstrakt:	Sensory neurons detect mechanical forces from both the environment and internal organs to regulate physiology. PIEZO2 is a mechanosensory ion channel critical for touch, proprioception, and bladder stretch sensation, yet its broad expression in sensory neurons suggests it has undiscovered physiological roles. To fully understand mechanosensory physiology, we must know where and when PIEZO2-expressing neurons detect force. The fluorescent styryl dye FM 1-43 was previously shown to label sensory neurons. Surprisingly, we find that the vast majority of FM 1-43 somatosensory neuron labeling in mice in vivo is dependent on PIEZO2 activity within the peripheral nerve endings. We illustrate the potential of FM 1-43 by using it to identify novel PIEZO2-expressing urethral neurons that are engaged by urination. These data reveal that FM 1-43 is a functional probe for mechanosensitivity via PIEZO2 activation in vivo and will facilitate the characterization of known and novel mechanosensory processes in multiple organ systems. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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