Functional response of Acinetobacter guillouiae SFC 500-1A to tannery wastewater as revealed by a complementary proteomic approach.

Autor: Fernandez M; Departamento de Biología Molecular, FCEFQyN, Universidad Nacional de Río Cuarto (UNRC), 5800, Río Cuarto, Córdoba, Argentina; Instituto de Biotecnología Ambiental y Salud (INBIAS-CONICET), Río Cuarto, Córdoba, Argentina. Electronic address: mfernandez@exa.unrc.edu.ar., Callegari EA; Division of Basic Biomedical Sciences, Sanford School of Medicine of the University of South Dakota, Vermillion, SD, USA. Electronic address: Eduardo.Callegari@usd.edu., Paez MD; Division of Basic Biomedical Sciences, Sanford School of Medicine of the University of South Dakota, Vermillion, SD, USA. Electronic address: Daniela.Paez@usd.edu., González PS; Departamento de Biología Molecular, FCEFQyN, Universidad Nacional de Río Cuarto (UNRC), 5800, Río Cuarto, Córdoba, Argentina; Instituto de Biotecnología Ambiental y Salud (INBIAS-CONICET), Río Cuarto, Córdoba, Argentina. Electronic address: pgonzalez@exa.unrc.edu.ar., Agostini E; Departamento de Biología Molecular, FCEFQyN, Universidad Nacional de Río Cuarto (UNRC), 5800, Río Cuarto, Córdoba, Argentina; Instituto de Biotecnología Ambiental y Salud (INBIAS-CONICET), Río Cuarto, Córdoba, Argentina. Electronic address: eagostini@exa.unrc.edu.ar.
Jazyk: angličtina
Zdroj: Journal of environmental management [J Environ Manage] 2023 Sep 15; Vol. 342, pp. 118333. Date of Electronic Publication: 2023 Jun 13.
DOI: 10.1016/j.jenvman.2023.118333
Abstrakt: Acinetobacter guillouiae SFC 500-1 A is a promising candidate for the bioremediation of tannery wastewater. In this study, we applied shotgun proteomic technology in conjunction with a gel-based assay (Gel-LC) to explore the strain's intracellular protein profile when grown in tannery wastewater as opposed to normal culture conditions. A total of 1775 proteins were identified, 52 of which were unique to the tannery wastewater treatment. Many of them were connected to the degradation of aromatic compounds and siderophore biosynthesis. On the other hand, 1598 proteins overlapped both conditions but were differentially expressed in each. Those that were upregulated in wastewater (109) were involved in the processes mentioned above, as well as in oxidative stress mitigation and intracellular redox state regulation. Particularly interesting were the downregulated proteins under the same treatment (318), which were diverse but mainly linked to the regulation of basic cellular functions (replication, transcription, translation, cell cycle, and wall biogenesis); metabolism (amino acids, lipids, sulphate, energetic processes); and other more complex responses (cell motility, exopolysaccharide production, biofilm formation, and quorum sensing). The findings suggest that SFC 500-1 A engages in survival and stress management strategies to cope with the toxic effects of tannery wastewater, and that such strategies may be mostly oriented at keeping metabolic processes to a minimum. Altogether, the results might be useful in the near future to improve the strain's effectiveness if it will be applied for bioremediation.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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