Modulatory influence of melatonin on apical periodontitis in Wistar rats fed a high-fat diet.

Autor: Santos RMD; São Paulo State University (UNESP), School of Dentistry, Araçatuba Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP) Araçatuba, Brazil; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas (PPGMCF)/Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Machado NEDS; São Paulo State University (UNESP), School of Dentistry, Araçatuba, Department of Preventive and Restorative Dentistry, Araçatuba, SP, Brazil., Cantiga-Silva C; São Paulo State University (UNESP), School of Dentistry, Araçatuba, Department of Preventive and Restorative Dentistry, Araçatuba, SP, Brazil., Belardi BE; São Paulo State University (UNESP), School of Dentistry, Araçatuba Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP) Araçatuba, Brazil; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas (PPGMCF)/Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Tsosura TVS; São Paulo State University (UNESP), School of Dentistry, Araçatuba Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP) Araçatuba, Brazil; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas (PPGMCF)/Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Chiba FY; São Paulo State University (UNESP), School of Dentistry, Araçatuba, Department of Preventive and Restorative Dentistry, Araçatuba, SP, Brazil., Tessarin GWL; São Paulo State University (UNESP), School of Dentistry, Araçatuba Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP) Araçatuba, Brazil; North University Center of São Paulo, Unorte, São José do Rio Preto, SP, Brazil. Electronic address: gestter@unorp.br., Mattera MSLC; São Paulo State University (UNESP), School of Dentistry, Araçatuba Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP) Araçatuba, Brazil; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas (PPGMCF)/Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Nobumoto ACTY; São Paulo State University (UNESP), School of Dentistry, Araçatuba Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP) Araçatuba, Brazil; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas (PPGMCF)/Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil., Ervolino E; São Paulo State University (UNESP), School of Dentistry, Araçatuba Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP) Araçatuba, Brazil., Cintra LTA; São Paulo State University (UNESP), School of Dentistry, Araçatuba, Department of Preventive and Restorative Dentistry, Araçatuba, SP, Brazil., Matsushita DH; São Paulo State University (UNESP), School of Dentistry, Araçatuba Department of Basic Sciences, School of Dentistry, São Paulo State University (UNESP) Araçatuba, Brazil; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas (PPGMCF)/Sociedade Brasileira de Fisiologia (SBFis), School of Dentistry, São Paulo State University (UNESP), Araçatuba, Brazil.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2023 Sep; Vol. 153, pp. 105749. Date of Electronic Publication: 2023 Jun 12.
DOI: 10.1016/j.archoralbio.2023.105749
Abstrakt: Objective: This study aimed to evaluate the influence of high-fat diet (HFD) and melatonin (MEL) treatment on the progression of inflammation and alveolar bone resorption (ABR) in rats with AP.
Design: Forty male Wistar rats were divided into four groups: apical periodontitis (AP), HFDAP, APMEL and HFDAPMEL. The animals were fed an HFD or standard diet for 107 days. On the 7th day, the rats were subjected to AP, and after 70 days, the rats in the MEL groups were treated with MEL for 30 days. Post treatment, the animals were euthanized, and their jaws were retrieved for evaluation of bone resorption, intensity of the inflammatory response, and immunohistochemical analysis including tartrate-resistant acid phosphatase (TRAP) and interleukin-1β (IL-1β) levels and tumor necrosis factor (TNF)-α expression.
Results: The APMEL group showed reduction in the inflammatory infiltrate and IL-1β expression relation to HFDAP, while the TNF-α levels did not differ among the groups. The HFDAP group showed an increase in the ABR. MEL reduced the TRAP levels in the APMEL and HFDAPMEL groups.
Conclusions: while MEL could reduce TRAP levels in the APMEL and HFDAPMEL groups, the reduction in the HFDAPMEL group was smaller than that in the APMEL group, demonstrating that the interaction between AP and HFD decreased the anti-resorptive effects of MEL.
Competing Interests: Declaration of Competing Interest The authors report no conflicts of interest related to this study.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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