Bacterial meningitis in the early postnatal mouse studied at single-cell resolution.

Autor:	Wang J; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, United States.; Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, United States., Rattner A; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, United States., Nathans J; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, United States.; Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, United States.; Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, United States.; Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2023 Jun 15; Vol. 12. Date of Electronic Publication: 2023 Jun 15.
DOI:	10.7554/eLife.86130
Abstrakt:	Bacterial meningitis is a major cause of morbidity and mortality, especially among infants and the elderly. Here, we study mice to assess the response of each of the major meningeal cell types to early postnatal E. coli infection using single nucleus RNA sequencing (snRNAseq), immunostaining, and genetic and pharamacologic perturbations of immune cells and immune signaling. Flatmounts of the dissected leptomeninges and dura were used to facilitiate high-quality confocal imaging and quantification of cell abundances and morphologies. Upon infection, the major meningeal cell types - including endothelial cells (ECs), macrophages, and fibroblasts - exhibit distinctive changes in their transcriptomes. Additionally, ECs in the leptomeninges redistribute CLDN5 and PECAM1, and leptomeningeal capillaries exhibit foci with reduced blood-brain barrier integrity. The vascular response to infection appears to be largely driven by TLR4 signaling, as determined by the nearly identical responses induced by infection and LPS administration and by the blunted response to infection in Tlr4 -/- mice. Interestingly, knocking out Ccr2 , encoding a major chemoattractant for monocytes, or acute depletion of leptomeningeal macrophages, following intracebroventricular injection of liposomal clodronate, had little or no effect on the response of leptomeningeal ECs to E. coli infection. Taken together, these data imply that EC responses to infection are largely driven by the intrinsic EC response to LPS. Competing Interests: JW, AR, JN No competing interests declared (© 2023, Wang et al.)
Databáze:	MEDLINE
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