Secondary Metabolite Analysis of Phomopsis sp. LGT-5 Based on the Dual Guidance of Whole-Genome Sequencing and HPLC-Q-ToF-MS/MS.

Autor: Zhu JJ; College of Pharmacy, Ningxia Medical University, Key Laboratory of Ningxia Minority Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, P. R. China.; Medical Science and Technology Research Center, Ningxia Medical University, Yinchuan, 750004, P. R. China., Xi N; Ningxia Rural Science and Technology Development Center, Science and Technology Department of Ningxia, Yinchuan, 750001, P. R. China., Liu C; Medical Science and Technology Research Center, Ningxia Medical University, Yinchuan, 750004, P. R. China., Ma PY; College of Pharmacy, Ningxia Medical University, Key Laboratory of Ningxia Minority Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, P. R. China., Wu XY; College of Pharmacy, Ningxia Medical University, Key Laboratory of Ningxia Minority Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, P. R. China., Zhou L; College of Pharmacy, Ningxia Medical University, Key Laboratory of Ningxia Minority Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, P. R. China., An ZY; College of Pharmacy, Ningxia Medical University, Key Laboratory of Ningxia Minority Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, P. R. China., Jiang ZB; School of Chemistry and Chemical Engineering, North Minzu University, Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, Yinchuan, 750021, P. R. China., Wu XL; College of Pharmacy, Ningxia Medical University, Key Laboratory of Ningxia Minority Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, P. R. China.; Medical Science and Technology Research Center, Ningxia Medical University, Yinchuan, 750004, P. R. China., Wang JH; College of Pharmacy, Ningxia Medical University, Key Laboratory of Ningxia Minority Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, P. R. China.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2023 Jul; Vol. 20 (7), pp. e202300275. Date of Electronic Publication: 2023 Jun 30.
DOI: 10.1002/cbdv.202300275
Abstrakt: Microorganisms produce a wealth of structurally diverse specialized metabolites with a remarkable range of biological activities. The Phomopsis sp. LGT-5 was obtained through tissue block and repeatedly crossed methods from Tripterygium wilfordii Hook. F. The antibacterial experiments of LGT-5 showed that it has high inhibitory activity against Staphylococcus aureus and Pseudomonas aeruginosa, and moderate inhibitory activity against Candida albicans. To research the generation of the antibacterial phenomenon of LGT-5 and provide support for further research and application, the whole genome sequencing (WGS) of LGT-5 was obtained by single-molecule real-time DNA sequencing platform Pacific Biosciences (PacBio) sequencing and Illumina paired-end sequencing. The final assembled LGT-5 genome is 54.79 Mb with a contig N50 of 290.07 kb; in addition, its secondary metabolites were detected through HPLC-Q-ToF-MS/MS. By comparing its MS/MS data, the secondary metabolites were analyzed based on visual network maps obtained on the Global Natural Products Social Molecular Networking (GNPS). The analysis results showed that the secondary metabolites of LGT-5 were triterpenes and various cyclic dipeptides.
(© 2023 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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