Development of High Throughput Microscope Mode Secondary Ion Mass Spectrometry Imaging.

Autor:	Green FM; The Rosalind Franklin Institute, Harwell Campus, Didcot OX11 0QX, U.K., Castellani ME; Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, U.K., Jia Y; Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, U.K., Eyres A; Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, U.K., Smith N; Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, U.K., Thompson S; Ionoptika Ltd, Unit B6 Millbrook Close, Chandler's Ford, Eastleigh SO53 4BZ, U.K., Blenkinsopp P; Ionoptika Ltd, Unit B6 Millbrook Close, Chandler's Ford, Eastleigh SO53 4BZ, U.K., Burt M; Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, U.K., Vallance C; Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, U.K., Bunch J; The Rosalind Franklin Institute, Harwell Campus, Didcot OX11 0QX, U.K., Takats Z; The Rosalind Franklin Institute, Harwell Campus, Didcot OX11 0QX, U.K., Brouard M; Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, U.K.
Jazyk:	angličtina
Zdroj:	Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2023 Jul 05; Vol. 34 (7), pp. 1272-1282. Date of Electronic Publication: 2023 Jun 15.
DOI:	10.1021/jasms.2c00371
Abstrakt:	This paper describes the development and initial results from a secondary ion mass spectrometer coupled with microscope mode detection. Stigmatic ion microscope imaging enables us to decouple the primary ion (PI) beam focus from spatial resolution and is a promising route to attaining higher throughput for mass spectrometry imaging (MSI). Using a commercial C 60 + PI beam source, we can defocus the PI beam to give uniform intensity across a 2.5 mm 2 area. By coupling the beam with a position-sensitive spatial detector, we can achieve mass spectral imaging of positive and negative secondary ions (SIs), which we demonstrate using samples comprising metals and dyes. Our approach involves simultaneous desorption of ions across a large field of view, enabling mass spectral images to be recorded over an area of 2.5 mm 2 in a matter of seconds. Our instrument can distinguish spatial features with a resolution of better than 20 μm, and has a mass resolution of >500 at 500 u. There is considerable scope to improve this, and through simulations we estimate the future performance of the instrument.
Databáze:	MEDLINE
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