Genome editing of a rice CDP-DAG synthase confers multipathogen resistance.

Autor: Sha G; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Sun P; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Kong X; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Han X; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Sun Q; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Fouillen L; Laboratoire de Biogenèse Membranaire, Université de Bordeaux, CNRS, Villenave-d'Ornon, France., Zhao J; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China.; College of Chemistry and Life Sciences, Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu, China., Li Y; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Yang L; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Wang Y; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Gong Q; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Zhou Y; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Zhou W; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Jain R; Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, USA.; Feedstocks Division, The Joint BioEnergy Institute, Emeryville, CA, USA., Gao J; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China., Huang R; National Engineering Research Center of Rice (Nanchang), Key Laboratory of Rice Physiology and Genetics of Jiangxi Province, Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, China., Chen X; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China.; College of Plant Protection, Anhui Agricultural University, Hefei, China., Zheng L; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Zhang W; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Qin Z; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China., Zhou Q; BGI-Shenzhen, Shenzhen, China., Zeng Q; State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, China., Xie K; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China., Xu J; Institute of Wetland Agriculture and Ecology, Shandong Academy of Agricultural Sciences, Jinan, China., Chiu TY; BGI-Shenzhen, Shenzhen, China., Guo L; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China., Mortimer JC; Feedstocks Division, The Joint BioEnergy Institute, Emeryville, CA, USA.; School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, South Australia, Australia., Boutté Y; Laboratoire de Biogenèse Membranaire, Université de Bordeaux, CNRS, Villenave-d'Ornon, France., Li Q; National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China., Kang Z; State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, China., Ronald PC; Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, USA. pcronald@ucdavis.edu.; Feedstocks Division, The Joint BioEnergy Institute, Emeryville, CA, USA. pcronald@ucdavis.edu.; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA. pcronald@ucdavis.edu., Li G; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China. li4@mail.hzau.edu.cn.; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China. li4@mail.hzau.edu.cn.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China. li4@mail.hzau.edu.cn.; The Center of Crop Nanobiotechnology, Huazhong Agricultural University, Wuhan, China. li4@mail.hzau.edu.cn.; Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, USA. li4@mail.hzau.edu.cn.; Feedstocks Division, The Joint BioEnergy Institute, Emeryville, CA, USA. li4@mail.hzau.edu.cn.
Jazyk: angličtina
Zdroj: Nature [Nature] 2023 Jun; Vol. 618 (7967), pp. 1017-1023. Date of Electronic Publication: 2023 Jun 14.
DOI: 10.1038/s41586-023-06205-2
Abstrakt: The discovery and application of genome editing introduced a new era of plant breeding by giving researchers efficient tools for the precise engineering of crop genomes 1 . Here we demonstrate the power of genome editing for engineering broad-spectrum disease resistance in rice (Oryza sativa). We first isolated a lesion mimic mutant (LMM) from a mutagenized rice population. We then demonstrated that a 29-base-pair deletion in a gene we named RESISTANCE TO BLAST1 (RBL1) caused broad-spectrum disease resistance and showed that this mutation caused an approximately 20-fold reduction in yield. RBL1 encodes a cytidine diphosphate diacylglycerol synthase that is required for phospholipid biosynthesis 2 . Mutation of RBL1 results in reduced levels of phosphatidylinositol and its derivative phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P 2 ). In rice, PtdIns(4,5)P 2 is enriched in cellular structures that are specifically associated with effector secretion and fungal infection, suggesting that it has a role as a disease-susceptibility factor 3 . By using targeted genome editing, we obtained an allele of RBL1, named RBL1 Δ12 , which confers broad-spectrum disease resistance but does not decrease yield in a model rice variety, as assessed in small-scale field trials. Our study has demonstrated the benefits of editing an LMM gene, a strategy relevant to diverse LMM genes and crops.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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