CD8+ T-cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis.

Autor:	García-López LL; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., Vargas-Montes M; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., Osorio-Méndez JF; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., Cardona N; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia.; Faculty of Dentistry, Universidad Antonio Nariño, Armenia, Quindío, Colombia., Hernández De Los Ríos A; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., Toro-Acevedo CA; Grupo Infección e Inmunidad, Faculty of Health Sciences, Universidad Tecnológica de Pereira, Pereira, Colombia., Arenas-García JC; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., Mantilla-Muriel LE; Grupo Infección e Inmunidad, Faculty of Health Sciences, Universidad Tecnológica de Pereira, Pereira, Colombia., Torres E; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., Valencia-Hernández JD; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., Acosta-Dávila A; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., de-la-Torre A; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia.; NeURos Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, Colombia., Celis-Giraldo D; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., Mejía Oquendo M; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia., Sepúlveda-Arias JC; Grupo Infección e Inmunidad, Faculty of Health Sciences, Universidad Tecnológica de Pereira, Pereira, Colombia., Gómez-Marín JE; GEPAMOL, Biomedical Research Center, Universidad del Quindío, Armenia, Quindío, Colombia.
Jazyk:	angličtina
Zdroj:	Ocular immunology and inflammation [Ocul Immunol Inflamm] 2024 Sep; Vol. 32 (7), pp. 1218-1227. Date of Electronic Publication: 2023 Jun 14.
DOI:	10.1080/09273948.2023.2217906
Abstrakt:	This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis ( n = 9), chronic asymptomatic toxoplasmosis ( n = 7), and non-infected people ( n = 5) by using RT-qPCR and flow cytometry techniques. The study found that gene expression of PD-1 and CD244, but not LAG-3, was higher in individuals with ocular toxoplasmosis versus individuals with asymptomatic infection or uninfected. Expression of PD1 in CD8+ central memory (CM) cells was higher in nine individuals with toxoplasmosis versus five uninfected individuals ( p = .003). After ex vivo stimulation, an inverse correlation was found between the exhaustion markers and quantitative clinical characteristics (lesion size, recurrence index, and number of lesions). A total exhaustion phenotype was found in 55.5% (5/9) of individuals with ocular toxoplasmosis. Our results suggest that the CD8+ exhaustion phenotype is involved in the pathogenesis of ocular toxoplasmosis.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu