Genomic adaptation of Burkholderia anthina to glyphosate uncovers a novel herbicide resistance mechanism.

Autor: Schwedt I; FG Synthetic Microbiology, Institute for Biotechnology, BTU Cottbus-Senftenberg, Senftenberg, Germany.; FG Molecular Microbiology, Institute of Biology, University of Hohenheim, Stuttgart, Germany., Collignon M; FG Synthetic Microbiology, Institute for Biotechnology, BTU Cottbus-Senftenberg, Senftenberg, Germany., Mittelstädt C; FG Synthetic Microbiology, Institute for Biotechnology, BTU Cottbus-Senftenberg, Senftenberg, Germany., Giudici F; FG Synthetic Microbiology, Institute for Biotechnology, BTU Cottbus-Senftenberg, Senftenberg, Germany., Rapp J; Interfaculty Institute for Microbiology and Infection Medicine Tübingen, University of Tübingen, Bacterial Metabolomics, Tübingen, Germany., Meißner J; Department of General Microbiology, Institute for Microbiology and Genetics, University of Goettingen, Göttingen, Germany., Link H; Interfaculty Institute for Microbiology and Infection Medicine Tübingen, University of Tübingen, Bacterial Metabolomics, Tübingen, Germany., Hertel R; FG Synthetic Microbiology, Institute for Biotechnology, BTU Cottbus-Senftenberg, Senftenberg, Germany.; Department of Genomic and Applied Microbiology, Institute for Microbiology and Genetics, University of Goettingen, Göttingen, Germany., Commichau FM; FG Synthetic Microbiology, Institute for Biotechnology, BTU Cottbus-Senftenberg, Senftenberg, Germany.; FG Molecular Microbiology, Institute of Biology, University of Hohenheim, Stuttgart, Germany.
Jazyk: angličtina
Zdroj: Environmental microbiology reports [Environ Microbiol Rep] 2023 Dec; Vol. 15 (6), pp. 727-739. Date of Electronic Publication: 2023 Jun 13.
DOI: 10.1111/1758-2229.13184
Abstrakt: Glyphosate (GS) specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase that converts phosphoenolpyruvate (PEP) and shikimate-3-phosphate to EPSP in the shikimate pathway of bacteria and other organisms. The inhibition of the EPSP synthase depletes the cell of the EPSP-derived aromatic amino acids as well as of folate and quinones. A variety of mechanisms (e.g., EPSP synthase modification) has been described that confer GS resistance to bacteria. Here, we show that the Burkholderia anthina strain DSM 16086 quickly evolves GS resistance by the acquisition of mutations in the ppsR gene. ppsR codes for the pyruvate/ortho-P i dikinase PpsR that physically interacts and regulates the activity of the PEP synthetase PpsA. The mutational inactivation of ppsR causes an increase in the cellular PEP concentration, thereby abolishing the inhibition of the EPSP synthase by GS that competes with PEP for binding to the enzyme. Since the overexpression of the Escherichia coli ppsA gene in Bacillus subtilis and E. coli did not increase GS resistance in these organisms, the mutational inactivation of the ppsR gene resulting in PpsA overactivity is a GS resistance mechanism that is probably unique to B. anthina.
(© 2023 The Authors. Environmental Microbiology Reports published by Applied Microbiology International and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje