Investigation of thymine as a potential cancer biomarker employing tryptophan with nanomaterials as a biosensor.

Autor: Abou El-Nour KM; Department of Chemistry, Suez Canal University, Faculty of Science, Ismailia 41522, Egypt. Electronic address: kabolnoor@yahoo.com., El-Sherbiny IM; Nanomedicine Research Labs, Center for Materials Sciences, Zewail City of Science and Technology, 6(th) October City, 12578 Giza, Egypt., Khairy GM; Department of Chemistry, Suez Canal University, Faculty of Science, Ismailia 41522, Egypt., Abbas AM; Department of Chemistry, Suez Canal University, Faculty of Science, Ismailia 41522, Egypt., Salem EH; Department of Chemistry, Suez Canal University, Faculty of Science, Ismailia 41522, Egypt.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2023 Nov 15; Vol. 301, pp. 122928. Date of Electronic Publication: 2023 Jun 07.
DOI: 10.1016/j.saa.2023.122928
Abstrakt: Tryptophan and tryptophan-based nanomaterials sensors in a solution have been developed to directly evaluate thymine. The determination of thymine has been done via quenching of the fluorescence of tryptophan and tryptophan-based nanomaterials such as graphene (Gr), graphene oxide (GO), gold nanoparticles (AuNPs), gold-silver nanocomposite (Au-Ag NC) in a physiological buffer. As the concentration of thymine rises, the fluorescence of tryptophan and tryptophan/nanomaterials becomes less intense. Trp, Trp/Gr, and tryptophan/(Au-Ag) NC systems' quenching mechanisms were dynamic, but tryptophan /GO and tryptophan/AuNPs' quenching mechanisms were static. The linear dynamic range for the determination of thy by tryptophan and tryptophan /nanomaterials is 10 to 200 μM. The detection limits for tryptophan, tryptophan /Gr, tryptophan /GO, tryptophan /AuNPs, and tryptophan/Au-Ag NC were 3.21, 14.20, 6.35, 4.67and 7.79 Μm, respectively. Thermodynamic parameters for the interaction of the Probes with Thy include the enthalpy (H°) and entropy (S°) change values, were assessed, as well as the binding constant (Ka) of Thy with Trp and Trp-based nanomaterials. A recovery study was conducted utilizing a human serum sample after the addition of the required quantity of the investigational thymine.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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