Differential Effects of Cocaine and Morphine on the Diurnal Regulation of the Mouse Nucleus Accumbens Proteome.

Autor:	Ketchesin KD; Translational Neuroscience Program, Department of Psychiatry, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, United States.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States., Becker-Krail DD; Translational Neuroscience Program, Department of Psychiatry, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, United States.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States., Xue X; Department of Biostatistics, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States., Wilson RS; Yale/NIDA Neuroproteomics Center, 300 George Street, New Haven, Connecticut 06511, United States.; W.M. Keck Biotechnology Mass Spectrometry (MS) & Proteomics Resource Laboratory, Yale University School of Medicine, New Haven, Connecticut 06511, United States.; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, United States., Lam TT; Yale/NIDA Neuroproteomics Center, 300 George Street, New Haven, Connecticut 06511, United States.; W.M. Keck Biotechnology Mass Spectrometry (MS) & Proteomics Resource Laboratory, Yale University School of Medicine, New Haven, Connecticut 06511, United States.; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, United States., Williams KR; Yale/NIDA Neuroproteomics Center, 300 George Street, New Haven, Connecticut 06511, United States.; Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, United States., Nairn AC; Yale/NIDA Neuroproteomics Center, 300 George Street, New Haven, Connecticut 06511, United States.; Department of Psychiatry, Yale University School of Medicine, Connecticut Mental Health Center, New Haven, Connecticut 06511, United States., Tseng GC; Department of Biostatistics, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States., Logan RW; Translational Neuroscience Program, Department of Psychiatry, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, United States.; Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, United States.; Department of Psychiatry, University of Massachusetts Chan Medical School, Worcester, Massachusetts 01655, United States.; Department of Neurobiology, University of Massachusetts Chan Medical School, Worcester, Massachusetts 01605, United States.
Jazyk:	angličtina
Zdroj:	Journal of proteome research [J Proteome Res] 2023 Jul 07; Vol. 22 (7), pp. 2377-2390. Date of Electronic Publication: 2023 Jun 13.
DOI:	10.1021/acs.jproteome.3c00126
Abstrakt:	Substance use disorders are associated with disruptions in sleep and circadian rhythms that persist during abstinence and may contribute to relapse risk. Repeated use of substances such as psychostimulants and opioids may lead to significant alterations in molecular rhythms in the nucleus accumbens (NAc), a brain region central to reward and motivation. Previous studies have identified rhythm alterations in the transcriptome of the NAc and other brain regions following the administration of psychostimulants or opioids. However, little is known about the impact of substance use on the diurnal rhythms of the proteome in the NAc. We used liquid chromatography coupled to tandem mass spectrometry-based quantitative proteomics, along with a data-independent acquisition analysis pipeline, to investigate the effects of cocaine or morphine administration on diurnal rhythms of proteome in the mouse NAc. Overall, our data reveal cocaine and morphine differentially alter diurnal rhythms of the proteome in the NAc, with largely independent differentially expressed proteins dependent on time-of-day. Pathways enriched from cocaine altered protein rhythms were primarily associated with glucocorticoid signaling and metabolism, whereas morphine was associated with neuroinflammation. Collectively, these findings are the first to characterize the diurnal regulation of the NAc proteome and demonstrate a novel relationship between the phase-dependent regulation of protein expression and the differential effects of cocaine and morphine on the NAc proteome. The proteomics data in this study are available via ProteomeXchange with identifier PXD042043.
Databáze:	MEDLINE
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