Identification of Differentiated Intestinal Epithelial Cells Using Immunostaining and Fluorescence Microscopy.

Autor: Digrazia JR; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA., Engevik MA; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA.; Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA., Engevik AC; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA. engevika@musc.edu.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2650, pp. 17-34.
DOI: 10.1007/978-1-0716-3076-1_2
Abstrakt: Immunofluorescence imaging enables visualization of a wide range of molecules in diverse cells and tissues. Determining the localization and endogenous protein levels in cells using immunostaining can be highly informative for researchers studying cell structure and function. The small intestinal epithelium is composed of numerous cell types including absorptive enterocytes, mucus-producing goblet cells, lysozyme positive Paneth cells, proliferative stem cells, chemosensing tuft cells, and hormone-producing enteroendocrine cells. Each cell type in the small intestine has unique functions and structures that are critical for maintaining intestinal homeostasis and identifiable by immunofluorescence labeling. In this chapter we provide a detailed protocol and representative images of immunostaining of paraffin-embedded mouse small intestinal tissue. The method highlights antibodies and micrographs that identify differentiated cell types. These details are important because quality immunofluorescence imaging can provide novel insights and a greater understanding of healthy and disease states.
(© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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