| Autor: |
Chen QQ; College of Tropical Crops, Hainan University, Haikou 570228, China., Wang ZJ; College of Tropical Crops, Hainan University, Haikou 570228, China., Chen YZ; College of Tropical Crops, Hainan University, Haikou 570228, China., Wang YQ; College of Tropical Crops, Hainan University, Haikou 570228, China., Zhu QL; College of Tropical Crops, Hainan University, Haikou 570228, China., Hu TY; College of Tropical Crops, Hainan University, Haikou 570228, China., Hu YJ; College of Tropical Crops, Hainan University, Haikou 570228, China., Wu YZ; College of Tropical Crops, Hainan University, Haikou 570228, China., Meng L; College of Tropical Crops, Hainan University, Haikou 570228, China., Tang SR; College of Tropical Crops, Hainan University, Haikou 570228, China. |
| Abstrakt: |
Biochar application may mitigate N 2 O emissions and increase crop yield, yet little is known about microbial dynamics variation. To investigate the potential of increasing yield and reducing emissions of biochar in tropical areas and the dynamic mechanism of related microorganisms, a pot experiment was conducted to investigate the biochar application on pepper yield, N 2 O emissions, and dynamic variation of related microorganisms. Three treatments were applied:2% biochar amendment (B), conventional fertilization (CON), and no nitrogen (CK). The results showed that the yield of the CON treatment was higher than that of the CK treatment. Compared with that of the CON treatment, biochar amendment significantly increased the yield of pepper by 18.0% ( P <0.05), and biochar amendment could increase the content of NH + 4 -N and NO - 3 -N in soil in most periods of pepper growth. Compared with that in the CON treatment, the B treatment significantly reduced cumulative N 2 O emissions by 18.3% ( P <0.05). Ammonia oxidizing archaea (AOA)- amoA and ammonia oxidizing bacteria (AOB)- amoA were very significantly negatively correlated with N 2 O flux ( P <0.01). N 2 O flux was significantly negatively correlated with nosZ gene abundance ( P <0.05). This indicated that N 2 O emission may have mainly resulted from the denitrification process. In the early stage of pepper growth, biochar significantly reduced N 2 O emissions by reducing the value of ( nirK + nirS )/ nosZ , whereas in the late stage of pepper growth, the value of ( nirK + nirS )/ nosZ of the B treatment was higher than that of the CON treatment, resulting in higher N 2 O flux in the B treatment. Therefore, biochar amendment could not only increase vegetable production in tropical areas but also reduce N 2 O emissions, which can be used as a new strategy to improve soil fertility in Hainan Province and other tropical areas. |
