Exploring plant cis-regulatory elements at single-cell resolution: overcoming biological and computational challenges to advance plant research.

Autor: Mendieta JP; Department of Genetics, University of Georgia, Athens, 30602, Georgia, USA., Sangra A; Department of Genetics, University of Georgia, Athens, 30602, Georgia, USA., Yan H; Department of Genetics, University of Georgia, Athens, 30602, Georgia, USA., Minow MAA; Department of Genetics, University of Georgia, Athens, 30602, Georgia, USA., Schmitz RJ; Department of Genetics, University of Georgia, Athens, 30602, Georgia, USA.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2023 Sep; Vol. 115 (6), pp. 1486-1499. Date of Electronic Publication: 2023 Jun 28.
DOI: 10.1111/tpj.16351
Abstrakt: Cis-regulatory elements (CREs) are important sequences for gene expression and for plant biological processes such as development, evolution, domestication, and stress response. However, studying CREs in plant genomes has been challenging. The totipotent nature of plant cells, coupled with the inability to maintain plant cell types in culture and the inherent technical challenges posed by the cell wall has limited our understanding of how plant cell types acquire and maintain their identities and respond to the environment via CRE usage. Advances in single-cell epigenomics have revolutionized the field of identifying cell-type-specific CREs. These new technologies have the potential to significantly advance our understanding of plant CRE biology, and shed light on how the regulatory genome gives rise to diverse plant phenomena. However, there are significant biological and computational challenges associated with analyzing single-cell epigenomic datasets. In this review, we discuss the historical and foundational underpinnings of plant single-cell research, challenges, and common pitfalls in the analysis of plant single-cell epigenomic data, and highlight biological challenges unique to plants. Additionally, we discuss how the application of single-cell epigenomic data in various contexts stands to transform our understanding of the importance of CREs in plant genomes.
(© 2023 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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