Plant-inspired adhesive and injectable natural hydrogels: in vitro and in vivo studies.

Autor: Bohórquez-Moreno CD; Department of Metallurgical & Materials Engineering, Faculty of Engineering, Sivas Cumhuriyet University, 58140, Sivas, Turkey., Öksüz KE; Department of Metallurgical & Materials Engineering, Faculty of Engineering, Sivas Cumhuriyet University, 58140, Sivas, Turkey. emre.oksuz@cumhuriyet.edu.tr., Dinçer E; Department of Nutrition & Dietetics, Faculty of Health Science, Sivas Cumhuriyet University, 58140, Sivas, Turkey., Hepokur C; Department of Biochemistry, Faculty of Pharmacy, Sivas Cumhuriyet University, 58140, Sivas, Turkey., Şen İ; Department of Surgery, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, 58140, Sivas, Turkey.
Jazyk: angličtina
Zdroj: Biotechnology letters [Biotechnol Lett] 2023 Sep; Vol. 45 (9), pp. 1209-1222. Date of Electronic Publication: 2023 Jun 13.
DOI: 10.1007/s10529-023-03400-z
Abstrakt: The development of alternative therapeutic treatments based on the use of medicinal and aromatic plants, such as Juniper communis L., has aroused interest in the medical field to find new alternatives to conventional therapeutic treatments, which have shown problems related to bacterial resistance, high costs, or sustainability in their production. The present work describes the use of hydrogels based on sodium alginate and carboxymethyl cellulose, with combinations of juniperus leaves and berry extracts, in order to characterize their chemical characteristics, antibacterial activity, tissue adhesion test, cytotoxicity in the L929 cell line, and their effects on an in vivo model in mice to maximize the use of these materials in the healthcare field. Overall, an adequate antibacterial potential against S. aureus, E. coli and P. vulgaris was obtained with doses above 100 mg.mL -1 of hydrogels. Likewise, low cytotoxicity in hydrogels combined with extracts has been identified according to the IC 50 value at 17.32 µg.mL -1 , compared to the higher cytotoxic activity expressed by the use of control hydrogels with a value at 11.05 µg.mL -1 . Moreover, in general, the observed adhesion was high to different tissues, showing its adequate capacity to be used in different tissue typologies. Furthermore, the invivo results have not shown erythema, edema, or other complications related to the use of the proposed hydrogels. These results suggest the feasibility of using these hydrogels in biomedical applications given the observed safety.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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